Linked Life Data

7527290

Source:http://linkedlifedata.com/resource/pubmed/id/7527290

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-1-9
pubmed:abstractText
Pharmacological characteristics of native neuronal nicotinic acetylcholine receptor-mediated ion currents in mouse N1E-115 neuroblastoma cells have been investigated by superfusion of voltage clamped cells with known concentrations of the agonists acetylcholine, nicotine and cytisine, and the antagonists alpha-bungarotoxin and neuronal bungarotoxin. The sensitivity of the nicotinic acetylcholine receptor for agonists followed the agonist potency rank-order: nicotine approximately acetylcholine >> cytisine. The EC50 values of acetylcholine and nicotine are 78 microM and 76 microM, respectively. Equal concentrations of acetylcholine and nicotine induce inward currents with approximately the same peak amplitude whereas cytisine induces much smaller inward currents. Acetylcholine-induced currents are unaffected by high concentrations of alpha-bungarotoxin. Conversely, at 10 and 90 nM neuronal bungarotoxin reduces the amplitude of the 1 mM acetylcholine-induced inward current to 47% and 11% of control values, respectively. Both the agonist potency rank-order and the differential sensitivity to snake toxins of nicotinic receptors in N1E-115 cells are consistent with the known pharmacological profile of alpha 4 beta 2 nicotinic receptors expressed in Xenopus oocytes and distinct from those of all other nicotinic acetylcholine receptors of known functional subunit compositions. All data indicate that the native nicotinic acetylcholine receptor in N1E-115 cells is an assembly of alpha 4 and beta 2 subunits, the putative major subtype of nicotinic acetylcholine receptor in the brain.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
654
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
312-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7527290-1-Methyl-3-isobutylxanthine, pubmed-meshheading:7527290-Acetylcholine, pubmed-meshheading:7527290-Alkaloids, pubmed-meshheading:7527290-Animals, pubmed-meshheading:7527290-Azocines, pubmed-meshheading:7527290-Bucladesine, pubmed-meshheading:7527290-Bungarotoxins, pubmed-meshheading:7527290-Cell Differentiation, pubmed-meshheading:7527290-Cell Line, pubmed-meshheading:7527290-Dose-Response Relationship, Drug, pubmed-meshheading:7527290-Kinetics, pubmed-meshheading:7527290-Macromolecular Substances, pubmed-meshheading:7527290-Membrane Potentials, pubmed-meshheading:7527290-Mice, pubmed-meshheading:7527290-Neuroblastoma, pubmed-meshheading:7527290-Neurons, pubmed-meshheading:7527290-Nicotine, pubmed-meshheading:7527290-Quinolizines, pubmed-meshheading:7527290-Receptors, Nicotinic, pubmed-meshheading:7527290-Tumor Cells, Cultured
pubmed:year
1994
pubmed:articleTitle
alpha 4 beta 2 subunit combination specific pharmacology of neuronal nicotinic acetylcholine receptors in N1E-115 neuroblastoma cells.
pubmed:affiliation
Research Institute of Toxicology, Utrecht University, The Netherlands.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't