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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
48
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pubmed:dateCreated |
1994-12-30
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pubmed:abstractText |
Besides having a role in signal transduction, heterotrimeric G proteins may also be involved in membrane trafficking events as suggested by their presence in specific intracellular compartments. In chromaffin cells, G alpha 0 is associated with secretory organelles, and its activation inhibits exocytosis. Although plasma membrane-bound G proteins are activated by cell-surface receptors, the intracellular proteins controlling organelle-associated G proteins are currently unknown. GAP-43, a neuronal protein enriched in axonal growth cones and presynaptic terminals, is one possible candidate since it can directly stimulate purified G0. We have investigated the interaction of adrenal medullary GAP-43 with chromaffin granule-associated G0 and its effect on catecholamine secretion. Cytosolic and depalmitoylated membrane-extracted GAP-43 were found to stimulate guanine nucleotide binding and exchange activity in chromaffin granule membranes. In permeabilized chromaffin cells, both forms of GAP-43 blocked calcium-dependent exocytosis, and this effect was inhibited by specific antibodies against G alpha 0. A synthetic peptide corresponding to the GAP-43 domain that interacts with G0 inhibited catecholamine secretion. This effect could be selectively reversed by the COOH-terminal peptide of G alpha 0. These results indicate that GAP-43 may be an endogenous pseudoreceptor for the secretory granule-bound form of G0 and can thereby control calcium-regulated exocytosis in chromaffin cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/GAP-43 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/GTP Phosphohydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Norepinephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Substance P,
http://linkedlifedata.com/resource/pubmed/chemical/Wasp Venoms,
http://linkedlifedata.com/resource/pubmed/chemical/mastoparan
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
269
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
30293-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7527027-Adrenal Medulla,
pubmed-meshheading:7527027-Animals,
pubmed-meshheading:7527027-Brain,
pubmed-meshheading:7527027-Cattle,
pubmed-meshheading:7527027-Cell Membrane,
pubmed-meshheading:7527027-Cells, Cultured,
pubmed-meshheading:7527027-Chickens,
pubmed-meshheading:7527027-Chromaffin Granules,
pubmed-meshheading:7527027-Dose-Response Relationship, Drug,
pubmed-meshheading:7527027-Exocytosis,
pubmed-meshheading:7527027-GAP-43 Protein,
pubmed-meshheading:7527027-GTP Phosphohydrolases,
pubmed-meshheading:7527027-GTP-Binding Proteins,
pubmed-meshheading:7527027-Growth Substances,
pubmed-meshheading:7527027-Homeostasis,
pubmed-meshheading:7527027-Kinetics,
pubmed-meshheading:7527027-Membrane Glycoproteins,
pubmed-meshheading:7527027-Nerve Tissue Proteins,
pubmed-meshheading:7527027-Norepinephrine,
pubmed-meshheading:7527027-Peptide Fragments,
pubmed-meshheading:7527027-Peptides,
pubmed-meshheading:7527027-Substance P,
pubmed-meshheading:7527027-Wasp Venoms
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pubmed:year |
1994
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pubmed:articleTitle |
GAP-43 controls the availability of secretory chromaffin granules for regulated exocytosis by stimulating a granule-associated G0.
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pubmed:affiliation |
Institut National de la Santé et de la Recherche Médicale, U-338 Biologie de la Communication Cellulaire, Strasbourg, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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