Linked Life Data

7526207

Source:http://linkedlifedata.com/resource/pubmed/id/7526207

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1994-12-22
pubmed:abstractText
Normative data were obtained on the kinetic properties of the acetylcholine receptor (AChR) channel at the human motor endplate by patch-clamp analysis. Single channel currents were recorded from 34 endplates of 8 nonweak subjects in the presence of 1 micron acetylcholine (ACh) at 22 +/- 0.5 degrees C. The vast majority of channels opened to a conductance of about 60 pS. The dwell-time distributions of these channels were well described as the sum of two exponential functions. The mean duration of the dominant longer component was 1.9 ms for the open intervals and 3.04 ms for the bursts. At three endplates, a small proportion of the channels had lower conductance and longer open time, resembling immature AChR channels. At 28 endplates it was also possible to obtain an estimate of the rate constant for channel closure (alpha) and approximate estimates for the rate constants of channel opening (beta) and ACh dissociation (k-2). Estimates of k-2 varied by 15% with methods of estimation. This is attributed to errors inherent in estimating the duration of the briefest channel events. The normative data will be useful for evaluating pathologic alterations in the kinetic properties of the AChR channel found in some congenital myasthenic syndromes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0148-639X
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1364-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Patch-clamp analysis of the properties of acetylcholine receptor channels at the normal human endplate.
pubmed:affiliation
Department of Neurology, Mayo Clinic, Rochester, Minnesota 55905.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't