Linked Life Data

7525943

Source:http://linkedlifedata.com/resource/pubmed/id/7525943

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-12-15
pubmed:abstractText
1. Intracellular Ca2+ concentration ([Ca2+]i) was monitored in single cells isolated from adult rat supraoptic (SO) nuclei. The great majority of cells (85%) were neurones and most were immunoreactive to oxytocin or to vasopressin (AVP). 2. The resting [Ca2+]i of the majority (80%) of the neurones remained stable while 20% of the neurones displayed spontaneous [Ca2+]i oscillations which disappeared in low-Ca2+ (100 nM) EGTA buffer. 3. Addition of 100 nM oxytocin increased the [Ca2+]i in both stable and oscillating cells. Two types of responses were observed: (i) a sustained response with [Ca2+]i being maintained at an elevated level and (ii) a brief response with [Ca2+]i quickly returning to a near-resting level. Responses were reproducible, dose dependent and blocked with a specific oxytocin antagonist. 4. Removal of extracellular Ca2+ did not block the oxytocin response. In EGTA buffer, application of thapsigargin (200 nM) onto oxytocin-sensitive cells induced an increase in [Ca2+]i and inhibited the oxytocin response. These effects were not induced by other intracellular Ca2+ mobilizers such as tBuBHQ (see Methods) or caffeine. 5. In conclusion, half of the SO cells respond to oxytocin with a rise in [Ca2+]i. The effect is mediated by oxytocin receptors and results from release of Ca2+ from thapsigargin-sensitive stores.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-1149842, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-1312449, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-1319014, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-1320457, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-1338107, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-1362442, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-1672455, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-1915840, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-2114320, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-2138778, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-2253774, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-2444888, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-2491804, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-2556386, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-2574199, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-2778722, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-2787587, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-2789101, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-2792248, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-2856095, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-3007746, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-3098302, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-3138987, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-3183714, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-3193957, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-3620965, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-3838314, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-3948915, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-6324027, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-6325126, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-6468562, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-6747898, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-7108799, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-7216614, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-7684897, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-8396014, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-8456910, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-8469313, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-8506027, http://linkedlifedata.com/resource/pubmed/commentcorrection/7525943-8518958
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/2,5-di-tert-butylhydroquinone, http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants, http://linkedlifedata.com/resource/pubmed/chemical/Arginine Vasopressin, http://linkedlifedata.com/resource/pubmed/chemical/Caffeine, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Transporting ATPases, http://linkedlifedata.com/resource/pubmed/chemical/Fura-2, http://linkedlifedata.com/resource/pubmed/chemical/Hydroquinones, http://linkedlifedata.com/resource/pubmed/chemical/Oxytocin, http://linkedlifedata.com/resource/pubmed/chemical/Terpenes, http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin, http://linkedlifedata.com/resource/pubmed/chemical/Vasotocin, http://linkedlifedata.com/resource/pubmed/chemical/vasotocin...
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-3751
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
478 ( Pt 2)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
275-87
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
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