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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1994-12-7
|
| pubmed:abstractText |
The intracerebral inoculation of Theiler's murine encephalomyelitis virus (TMEV) into susceptible strains of mice results in a chronic, immune-mediated demyelinating disease that shares many features with human multiple sclerosis. As with human MS, T lymphocytes seem to be critically important for the pathogenesis of this virally induced, demyelinating disease. Therefore, determining the fine specificity of the T cell response may be essential for elucidating the mechanism(s) involved in demyelination. By using fusion proteins and synthetic peptides, we have initially identified a region within the amino acid residues 233 to 250 of the VP1 capsid protein of Theiler's virus that is recognized by T cells from either TMEV-immunized or TMEV-infected, demyelination-susceptible SJL/J mice. A T lymphocyte precursor frequency analysis indicates that a major TMEV-reactive T cell population in the periphery of virus-infected mice recognizes this VP1 region. The fine epitope specificity has been further determined to be within VP1(233-244) using additional synthetic peptides. VP1(233-244)-specific T cells seem to represent a significant population of TMEV-reactive T lymphocytes within the demyelinating lesions, because such T cells have been cloned from the spinal cords of infected mice. Interestingly, all TMEV-specific T cell clones derived from the demyelinating lesions, regardless of epitope specificity, produce IFN-gamma on stimulation and thus may play a critical role in the recruitment and activation of inflammatory cells leading to demyelination. Taken together, these data suggest that a T cell response against VP1(233-244) is involved in the pathogenesis of TMEV-induced demyelinating disease.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Capsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/VP1 protein, Theilovirus
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
153
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4508-19
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7525707-Animals,
pubmed-meshheading:7525707-Antigens, Viral,
pubmed-meshheading:7525707-Base Sequence,
pubmed-meshheading:7525707-Blotting, Western,
pubmed-meshheading:7525707-Capsid,
pubmed-meshheading:7525707-Capsid Proteins,
pubmed-meshheading:7525707-Cytokines,
pubmed-meshheading:7525707-Demyelinating Diseases,
pubmed-meshheading:7525707-Epitopes,
pubmed-meshheading:7525707-Indicator Dilution Techniques,
pubmed-meshheading:7525707-Lymphocyte Activation,
pubmed-meshheading:7525707-Mice,
pubmed-meshheading:7525707-Mice, Inbred Strains,
pubmed-meshheading:7525707-Molecular Sequence Data,
pubmed-meshheading:7525707-Multiple Sclerosis,
pubmed-meshheading:7525707-Poliomyelitis,
pubmed-meshheading:7525707-Recombinant Fusion Proteins,
pubmed-meshheading:7525707-T-Lymphocytes,
pubmed-meshheading:7525707-Theilovirus
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
A predominant viral epitope recognized by T cells from the periphery and demyelinating lesions of SJL/J mice infected with Theiler's virus is located within VP1(233-244).
|
| pubmed:affiliation |
Department of Pathology, Northwestern University Medical School, Chicago, IL 60611.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|