Linked Life Data

7525476

Source:http://linkedlifedata.com/resource/pubmed/id/7525476

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1994-12-2
pubmed:abstractText
Recent studies have revealed that angiotensin II (Ang II) interacts with two pharmacologically different types of seven-transmembrane domain receptors, hence named Ang II type 1 and type 2 (AT1 and AT2) receptors. cDNAs for the AT1 receptor have been cloned, and the existence of two receptor subtypes, AT1A and AT1B, has been revealed in rat and mouse. This study presents a new approach for the specific quantification of AT1A and AT1B receptor mRNAs by reverse transcription and polymerase chain reaction amplification in the presence of an AT1 receptor mutant cRNA as internal standard. Absolute quantities of mRNA are then determined by extrapolation using the standard curve generated with the internal standard. Moreover, addition of this internal standard to each tube controls for both reverse transcription and polymerase chain reaction amplification in each sample. In male Wistar rats, the highest absolute AT1A receptor mRNA levels were found in liver and kidney and those for AT1B receptor mRNA in the pituitary. Expressed as a percentage of total AT1A+AT1B receptor mRNA content, AT1A receptor mRNA content was 100% in liver, 85% in lung, 73% in kidney, 65% in aorta, 48% in adrenals, and 15% in the hypophysis. Since this approach can determine absolute AT1A and AT1B receptor mRNA quantities in different organs, it allows the study of the regulation of their expression under different pathophysiological conditions. After sodium depletion, known to induce hyperactivity of the renin-angiotensin system, adrenal AT1A and AT1B receptor mRNA levels were increased by 60% and 110%, respectively. In contrast, in renovascular hypertension (two-kidney, one clip), also associated with elevated circulating plasma renin activity, adrenal AT1B receptor mRNA levels decreased by 50%, whereas there was no change in those of AT1A. Therefore, the differential distribution and regulation of these two receptor subtypes suggest that each of them might be involved in the mediation of different biological effects of Ang II.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0194-911X
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
538-48
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7525476-Adrenal Glands, pubmed-meshheading:7525476-Analysis of Variance, pubmed-meshheading:7525476-Angiotensin II, pubmed-meshheading:7525476-Animals, pubmed-meshheading:7525476-Base Sequence, pubmed-meshheading:7525476-DNA Primers, pubmed-meshheading:7525476-Diet, Sodium-Restricted, pubmed-meshheading:7525476-Gene Expression, pubmed-meshheading:7525476-Gene Expression Regulation, pubmed-meshheading:7525476-Hypertension, Renovascular, pubmed-meshheading:7525476-Kinetics, pubmed-meshheading:7525476-Liver, pubmed-meshheading:7525476-Male, pubmed-meshheading:7525476-Mice, pubmed-meshheading:7525476-Molecular Sequence Data, pubmed-meshheading:7525476-Organ Specificity, pubmed-meshheading:7525476-Polymerase Chain Reaction, pubmed-meshheading:7525476-RNA, Messenger, pubmed-meshheading:7525476-RNA-Directed DNA Polymerase, pubmed-meshheading:7525476-Radioligand Assay, pubmed-meshheading:7525476-Rats, pubmed-meshheading:7525476-Rats, Wistar, pubmed-meshheading:7525476-Receptors, Angiotensin, pubmed-meshheading:7525476-Reference Values
pubmed:year
1994
pubmed:articleTitle
Tissular expression and regulation of type 1 angiotensin II receptor subtypes by quantitative reverse transcriptase-polymerase chain reaction analysis.
pubmed:affiliation
INSERM U36, Collège de France, Paris.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't