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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1994-11-28
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pubmed:abstractText |
Membrane currents produced by the expression of a rat GABA transporter (GAT-1) stably transfected into HEK293 cells were characterized with a whole-cell voltage clamp. Three modes of function were identified: ex-gated currents produced by extracellular GABA, in-gated currents produced by intracellular GABA, and uncoupled currents produced in the absence of GABA. The ex-gated current was not the reversal of the in-gated current; moreover, the stoichiometry between GABA and co-ions was not always fixed. Each mode of function required a different set of ions on the two sides of the membrane. We made rapid solution changes and observed an allosteric effect of Na+ that only occurred at the extracellular surface. Thus, the GAT-1 transporter does not behave like a recirculating carrier but may be described as a pore with ion gates at either end that are controlled in part by allosteric sites.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0896-6273
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
949-60
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7524562-Allosteric Site,
pubmed-meshheading:7524562-Animals,
pubmed-meshheading:7524562-Base Sequence,
pubmed-meshheading:7524562-Biological Transport,
pubmed-meshheading:7524562-Carrier Proteins,
pubmed-meshheading:7524562-Cell Line,
pubmed-meshheading:7524562-Electric Conductivity,
pubmed-meshheading:7524562-GABA Plasma Membrane Transport Proteins,
pubmed-meshheading:7524562-Ion Channel Gating,
pubmed-meshheading:7524562-Ion Channels,
pubmed-meshheading:7524562-Membrane Potentials,
pubmed-meshheading:7524562-Membrane Proteins,
pubmed-meshheading:7524562-Membrane Transport Proteins,
pubmed-meshheading:7524562-Molecular Sequence Data,
pubmed-meshheading:7524562-Organic Anion Transporters,
pubmed-meshheading:7524562-Rats,
pubmed-meshheading:7524562-Sodium,
pubmed-meshheading:7524562-Thermodynamics,
pubmed-meshheading:7524562-Transfection,
pubmed-meshheading:7524562-gamma-Aminobutyric Acid
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pubmed:year |
1994
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pubmed:articleTitle |
A GABA transporter operates asymmetrically and with variable stoichiometry.
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pubmed:affiliation |
Department of Pharmacological and Physiological Sciences, University of Chicago, Illinois 60637.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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