|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1994-11-23
|
|
pubmed:abstractText |
We describe the negative regulatory activity of a 1.7 kilobase (kb) region (R) in the human beta-globin locus located between 4.0 and 2.3 kb upstream of the delta-globin gene capsite, using a transient assay with the chloramphenicol acetyltransferase (CAT) reporter gene in mouse erythroleukemia (MEL) cells. The R region is deleted in most cases of deletion hereditary persistence of fetal hemoglobin (HPFH), but is unaffected in most delta beta zero-thalassemias. However, no experiments addressing its function in globin gene expression have been reported to date. We show that R inhibits CAT expression of constructs containing a fetal (gamma) or adult (beta) globin gene promoter, but does not affect expression of similar constructs using a non-globin (SV40) promoter. The inhibitory effect on the beta-globin promoter can be localized to a 651 bp sub-region of R. For the gamma-globin promoter, no sub-region of R can reproduce the level of inhibition associated with the entire region.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0006-291X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
14
|
|
pubmed:volume |
204
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
413-8
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:7524502-Animals,
pubmed-meshheading:7524502-Base Sequence,
pubmed-meshheading:7524502-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:7524502-DNA Primers,
pubmed-meshheading:7524502-Fetal Hemoglobin,
pubmed-meshheading:7524502-Genes, Regulator,
pubmed-meshheading:7524502-Globins,
pubmed-meshheading:7524502-Hemoglobin A,
pubmed-meshheading:7524502-Hemoglobinopathies,
pubmed-meshheading:7524502-Humans,
pubmed-meshheading:7524502-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:7524502-Mice,
pubmed-meshheading:7524502-Molecular Sequence Data,
pubmed-meshheading:7524502-Polymerase Chain Reaction,
pubmed-meshheading:7524502-Promoter Regions, Genetic,
pubmed-meshheading:7524502-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:7524502-Sequence Deletion,
pubmed-meshheading:7524502-Transfection,
pubmed-meshheading:7524502-Tumor Cells, Cultured,
pubmed-meshheading:7524502-beta-Thalassemia
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
Evidence for a globin promoter-specific silencer element located upstream of the human delta-globin gene.
|
|
pubmed:affiliation |
Unita di Ricerca Piera Cutino, Ospedale V. Cervello, Palermo, Italy.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
