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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4 Pt 1
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pubmed:dateCreated |
1994-11-22
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pubmed:abstractText |
We studied the effects of exogenous nitric oxide (NO) on leukocyte-endothelial interaction after 60 min of splanchnic artery ischemia and 120 min of reperfusion (SAO/R) in pentobarbital sodium-anesthetized rats via intravital microscopy. Treatment with the NO donor S-nitroso-N-acetylpenicillamine (SNAP, 20 micrograms/kg bolus followed by infusion at 20 micrograms.kg-1.h-1), beginning 10 min before reperfusion, resulted in significantly decreased leukocyte-endothelial interaction. This was manifested by a significant decrease in leukocyte rolling and adherence in the postcapillary venules. Tissue protection was demonstrated by a significantly lower plasma free amino-nitrogen concentration in the SNAP-treated SAO/R rats compared with those receiving NO-depleted SNAP (P < 0.05). Immunohistochemical localization of P-selectin showed significantly decreased P-selectin expression on the venular endothelium after SAO/R in rats given SNAP 10 min before reperfusion (23.0 +/- 3.2% vs. 54.9 +/- 12.1% positive staining, respectively, P < 0.01). From these data, we conclude that the effects of exogenous NO on leukocyte-endothelial interaction after ischemia-reperfusion appear to be at least partially mediated through the endothelial adhesion molecule P-selectin.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/P-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Penicillamine,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/S-Nitroso-N-Acetylpenicillamine,
http://linkedlifedata.com/resource/pubmed/chemical/Vasodilator Agents
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0002-9513
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
G562-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:7524346-Animals,
pubmed-meshheading:7524346-Cell Adhesion,
pubmed-meshheading:7524346-Endothelium, Vascular,
pubmed-meshheading:7524346-Hemodynamics,
pubmed-meshheading:7524346-Immunohistochemistry,
pubmed-meshheading:7524346-Ischemia,
pubmed-meshheading:7524346-Leukocytes,
pubmed-meshheading:7524346-Male,
pubmed-meshheading:7524346-Nitric Oxide,
pubmed-meshheading:7524346-P-Selectin,
pubmed-meshheading:7524346-Penicillamine,
pubmed-meshheading:7524346-Platelet Membrane Glycoproteins,
pubmed-meshheading:7524346-Rats,
pubmed-meshheading:7524346-Rats, Sprague-Dawley,
pubmed-meshheading:7524346-Reperfusion,
pubmed-meshheading:7524346-S-Nitroso-N-Acetylpenicillamine,
pubmed-meshheading:7524346-Splanchnic Circulation,
pubmed-meshheading:7524346-Vasodilator Agents
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pubmed:year |
1994
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pubmed:articleTitle |
Nitric oxide attenuates leukocyte-endothelial interaction via P-selectin in splanchnic ischemia-reperfusion.
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pubmed:affiliation |
Department of Physiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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