7524081

Source:http://linkedlifedata.com/resource/pubmed/id/7524081

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0002199, umls-concept:C0035820, umls-concept:C0037083, umls-concept:C0330390, umls-concept:C0337112, umls-concept:C0597357, umls-concept:C0678594, umls-concept:C1167322, umls-concept:C1523873, umls-concept:C1524075, umls-concept:C1710082, umls-concept:C2752508
pubmed:issue	20
pubmed:dateCreated	1994-10-27
pubmed:abstractText	A previously cloned cDNA encodes one subunit of the human interferon alpha/beta receptor (IFN alpha R), denoted IFN alpha R1. To study the expression and signaling of IFN alpha R1, we used monoclonal antibodies (mAbs) generated against the baculovirus-expressed ectodomain of IFN alpha R1. Immunoprecipitation and immunoblotting of lysates from a variety of human cell lines showed that IFN alpha R1 has an apparent molecular mass of 135 kDa. Binding analysis with 125I-labeled mAb demonstrated high levels of cell surface expression of IFN alpha R1 in human cells and in mouse cells transfected with IFN alpha R1 cDNA, whereas no cross-reactivity was observed in control mouse L929 cells expressing only the endogenous mouse receptor. The subunit was rapidly down-regulated by IFN alpha (80% decrease within 2 hr) and degraded upon internalization. The IFN alpha R1 chain appeared to be constitutively associated with the 115-kDa subunit of the IFN alpha/beta receptor, since the mAbs coprecipitated this protein. IFN alpha/beta treatment induced tyrosine phosphorylation of IFN alpha R1 within 1 min, with kinetics paralleling that of the IFN-activated protein-tyrosine kinases Jak1 and Tyk2. Ligand-induced tyrosine phosphorylation of IFN alpha R1 was blocked by the kinase inhibitors genistein or staurosporine. Although IFN alpha R1 cDNA-transfected mouse cells expressed high levels of this subunit when compared with empty vector-transfected cells the number of binding sites for human IFN alpha (50-75 sites per cell) was not increased. Human IFN alpha induced the expression of a mouse IFN alpha/beta-responsive gene (the 204 gene) in mouse L929 cells transfected with the IFN alpha R1 cDNA, but not in mock-transfected cells. These results suggest that the IFN alpha R1 subunit acts as a species-specific signal transduction component of the IFN alpha/beta receptor complex.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/GM36716
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-1334470, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-1386289, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-1446745, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-1496401, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-1531999, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-1533935, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-1638633, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-1690559, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-1832772, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-1956339, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-2153461, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-2168551, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-2169613, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-2466092, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-2477366, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-2953024, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-3476954, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-3990797, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-6292184, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-7689979, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-8124716, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-8124717, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-8144546, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-8181059, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-8232552, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-8272873, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-8343951, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-8346200, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-8366117, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-8395346, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-8423335, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-8468477, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524081-8477442
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/7505876
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal, http://linkedlifedata.com/resource/pubmed/chemical/Interferon-alpha, http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine, http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Interferon alpha-beta, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interferon, http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
pubmed:status	MEDLINE
pubmed:month	Sep
pubmed:issn	0027-8424
pubmed:author	pubmed-author:BattRR, pubmed-author:ConstantinescuS NSN, pubmed-author:CrozeEE, pubmed-author:MullersmanJ EJE, pubmed-author:MurtiAA, pubmed-author:PfefferL MLM, pubmed-author:WangCC
pubmed:issnType	Print
pubmed:day	27
pubmed:volume	91
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	9602-6
pubmed:dateRevised	2009-11-18
pubmed:meshHeading	pubmed-meshheading:7524081-Animals, pubmed-meshheading:7524081-Antibodies, Monoclonal, pubmed-meshheading:7524081-Cell Line, pubmed-meshheading:7524081-Cell Membrane, pubmed-meshheading:7524081-Gene Expression, pubmed-meshheading:7524081-HeLa Cells, pubmed-meshheading:7524081-Humans, pubmed-meshheading:7524081-Interferon-alpha, pubmed-meshheading:7524081-Macromolecular Substances, pubmed-meshheading:7524081-Membrane Proteins, pubmed-meshheading:7524081-Mice, pubmed-meshheading:7524081-Phosphorylation, pubmed-meshheading:7524081-Phosphotyrosine, pubmed-meshheading:7524081-Receptor, Interferon alpha-beta, pubmed-meshheading:7524081-Receptors, Interferon, pubmed-meshheading:7524081-Signal Transduction, pubmed-meshheading:7524081-Transfection, pubmed-meshheading:7524081-Tumor Cells, Cultured, pubmed-meshheading:7524081-Tyrosine
pubmed:year	1994
pubmed:articleTitle	Role of interferon alpha/beta receptor chain 1 in the structure and transmembrane signaling of the interferon alpha/beta receptor complex.

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