Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
1994-11-23
pubmed:abstractText
The Drosophila sex-lethal (Sxl) protein, a regulator of somatic sexual differentiation, is an RNA binding protein with two potential RNA recognition motifs (RRMs). It is thought to exert its function on splicing by binding to specific RNA sequences within Sxl and transformer (tra) pre-mRNAs. To examine the Sxl RNA binding specificity in detail, we performed in vitro selection and amplification of ligand RNAs from a random sequence pool on the basis of affinity with Sxl protein. After three cycles of selection and amplification, we cloned and sequenced 17 cDNAs corresponding to the RNAs selected in vitro. Sequencing showed that most of the RNAs selected contain polyuridine stretches surrounded by purine residues. In vitro binding analysis revealed that the sequences of the in vitro selected RNAs with relatively high affinity for Sxl show similarity to that of the Sxl- and tra-regulated acceptor regions, including the invariant AG sequence for splicing. These results suggest that Sxl recognizes and preferentially binds to a polyuridine stretch with a downstream AG sequence.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-1454517, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-1690860, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-1696729, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-1716386, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-1717938, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-1839712, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-2015624, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-2112300, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-2200121, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-2467746, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-2503251, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-2505080, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-2522817, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-2647303, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-2680106, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-3072706, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-3144435, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-3537727, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-4075405, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-7510636, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-7516476, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-7680770, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-7926751, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-8246990, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-8276242, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-8325488, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-8340391, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-8441386, http://linkedlifedata.com/resource/pubmed/commentcorrection/7524034-8449402
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4082-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Characterization of RNA binding specificity of the Drosophila sex-lethal protein by in vitro ligand selection.
pubmed:affiliation
Department of Biology, Faculty of Science, Kobe University, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't