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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1994-11-16
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pubmed:abstractText |
Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524029-1283705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524029-1421515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524029-3002422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524029-3277187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524029-3684574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524029-5418456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524029-6091052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524029-6313211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524029-6765237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524029-7688451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524029-8155898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524029-8219322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524029-8329766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524029-8341593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7524029-8457722
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3866-70
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1994
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pubmed:articleTitle |
Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa.
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pubmed:affiliation |
Department of Molecular Biology, Odense University, Denmark.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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