Linked Life Data

7523654

Source:http://linkedlifedata.com/resource/pubmed/id/7523654

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-11-7
pubmed:abstractText
Lindane (gamma-hexachlorocyclohexane) is an organochlorine pesticide that increases intracellular free calcium ([Ca++]i) in several tissues. Calcium homeostasis is central to the excitation and relaxation of uterine muscle during labor. The present study, therefore, investigated whether lindane exposure modulated [Ca++]i in myometrial smooth muscle cells. This study demonstrated that lindane, but not beta-hexachlorocyclohexane, increased [Ca++]i in a concentration-dependent manner in individual rat myometrial cells, as measured with the calcium-sensitive probe fura-2-AM. The lindane-induced Ca++ response was rapid in onset and protracted in duration. The lindane response was apparently independent of external calcium because equivalent [Ca++]i responses were observed in cells exposed to lindane in Ca(++)-containing and Ca(++)-free media and in the presence of 10 microM nifedipine, a dihydropyridine blocker of plasma membrane voltage-sensitive Ca++ channels. Prior depletion of internal Ca++ stores that contained Ca(++)-induced Ca(++)-release channels by 10 mM caffeine and 1 microM ryanodine did not affect lindane's ability to increase [Ca++]i, whereas pretreatment with either 1 microM ionomycin or 5 microM carbachol eliminated the lindane-induced [Ca++]i increase. These experiments suggest that lindane increases [Ca++]i through the selective release of inositol 1,4,5-trisphosphate-sensitive Ca++ stores. In addition, in Ca(++)-containing buffer, low concentrations of lindane (1 microM) rapidly inhibited the regenerative calcium oscillations induced by carbachol. If the mechanism is similar in vivo, lindane exposure may perturb many finely regulated Ca(++)-dependent processes required for excitation-contraction coupling in successful parturition and, therefore, increase the probability of delayed or dysfunctional labor.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-3565
pubmed:author
pubmed:issnType
Print
pubmed:volume
270
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1015-24
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Lindane increases intracellular calcium in rat myometrial smooth muscle cells through modulation of inositol 1,4,5-trisphosphate-sensitive stores.
pubmed:affiliation
Department of Environmental and Industrial Health, University of Michigan, Ann Arbor.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.