Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1994-11-10
|
| pubmed:abstractText |
A rat mAb termed 1B11 recognizes a 130-kDa cell surface glycoprotein expressed on T lymphocytes. Transfection studies using the Cd43 gene transfected into murine L cells, and immunoblots using anti-peptide Abs specific for the CD43 polypeptide identified the 1B11 Ag as the 130-kDa isoform of murine CD43. mAb 1B11 fails to recognize the other major CD43 isoform, 115-kDa CD43, either by Western blotting or by FACS analysis, thus differing from the previously characterized anti-CD43 mAb S7 that recognizes only the CD43 115-kDa isoform and not the CD43 130-kDa isoform. CD43 130-kDa recognized by mAb 1B11 is differentially expressed on T lymphocytes. Whereas most CD4-8-, CD4+8+, and CD4-8+ thymocytes express 130-kDa CD43 constitutively, the Ag is expressed by less than 20% of CD4+ T cells in immature and mature populations. On activation, expression of 130-kDa CD43 is up-regulated dramatically on CD4+ T lymphocytes, and to a lesser extent on CD8+ T lymphocytes. In contrast, T cell activation resulted in only minor up-regulation of 115-kDa CD43. CD43 130-kDa contains sialylated O-linked carbohydrate; however, recognition by mAb 1B11 is not dependent on the presence of sialic acid. Interestingly, removal of sialic acid by neuraminidase treatment of 1B11-negative CD4+ T lymphocytes or 1B11-negative EL4 cells confers 1B11 reactivity, suggesting that the 1B11 epitope is masked by sialic acid residues on the CD43 115-kDa isoform. The isoelectric point (pl) of 130-kDa CD43 was determined to be 6.0, which is higher than the pl reported for 115-kDa CD43. Different molecular properties of 115-kDa and 130-kDa CD43 and their differential expression in T cell subsets may indicate specific roles for these CD43 isoforms in T cell ontogeny and/or T cell function.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD43,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Neuraminidase,
http://linkedlifedata.com/resource/pubmed/chemical/Sialic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Spn protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Spn protein, rat
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
153
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3426-39
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7523493-Animals,
pubmed-meshheading:7523493-Antibodies, Monoclonal,
pubmed-meshheading:7523493-Antigens, CD,
pubmed-meshheading:7523493-Antigens, CD43,
pubmed-meshheading:7523493-CD4-Positive T-Lymphocytes,
pubmed-meshheading:7523493-CD8-Positive T-Lymphocytes,
pubmed-meshheading:7523493-Cell Line,
pubmed-meshheading:7523493-Epitopes,
pubmed-meshheading:7523493-Lymphocyte Activation,
pubmed-meshheading:7523493-Lymphoid Tissue,
pubmed-meshheading:7523493-Mice,
pubmed-meshheading:7523493-Mice, Inbred Strains,
pubmed-meshheading:7523493-Molecular Weight,
pubmed-meshheading:7523493-Neuraminidase,
pubmed-meshheading:7523493-Rats,
pubmed-meshheading:7523493-Rats, Wistar,
pubmed-meshheading:7523493-Sialic Acids,
pubmed-meshheading:7523493-Sialoglycoproteins,
pubmed-meshheading:7523493-T-Lymphocyte Subsets
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Characterization of the activation-associated isoform of CD43 on murine T lymphocytes.
|
| pubmed:affiliation |
Biomedical Research Center, Vancouver, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|