Linked Life Data

7523169

Source:http://linkedlifedata.com/resource/pubmed/id/7523169

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1994-11-10
pubmed:abstractText
We determined the appropriate incubation period to expand human peripheral blood (PB) CD34+ cells for clinical application and the role of recombinant human (rh) interleukin-3 (rhIL-3) in the expansion and differentiation of these cells. The cells were purified up to 40 +/- 16% and transitional changes in immunophenotypic subpopulations in suspension culture were examined following stimulation with three different combinations of rh colony-stimulating factors (rhCSFs): 1) rhIL-3 alone, 2) rhIL-6, rhSCF, rhG-CSF, plus rhGM-CSF, and 3) the four CSFs plus rhIL-3. With all three CSF combinations, the total cells increased continuously after day 5 until day 14, and a combination of the five CSFs always gave rise to the highest number of total cells. Immunophenotypic analysis of the expanded cells showed that with all three CSF combinations CD34+ cells reached a maximal rate on day 5 and then decreased in an inverse correlation between the logarithm of CD34 positive rate and the duration of suspension culture. The maximum expansion of CD34+ cells and PB progenitor cells (PBPC) with rhIL-3 alone, the four CSFs, or the five CSFs was observed on day 5, 10, or 7, respectively. The combination of the five CSFs was identified as the most potent stimulus for expansion of PBPC and CD34+ cells, as it increased non-erythroid PBPC 89 +/- 69-fold, with a range of 24 to 204-fold on day 7. However, differences in the expansion rate of these cells on days 5, 7, and 10 were not statistically significant. The majority of purified CD34+ cells coexpressed CD38 (91 +/- 3%) but were negative for CD33 (85 +/- 4%), and one-half coexpressed CD13. With all three combinations of CSFs, the mature CD34+ cells that coexpressed CD38, CD33, or CD13 expanded in parallel with the total CD34+ cells, while an increase in relatively immature CD34+ cells, which do not express CD38, CD33, or CD13, was only statistically significant with the five CSFs. Thus, rhIL-3 played a critical role when combined with the four CSFs by increasing both mature and immature CD34+ cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0301-472X
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1171-8
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:7523169-Adult, pubmed-meshheading:7523169-Antigens, CD, pubmed-meshheading:7523169-Antigens, CD34, pubmed-meshheading:7523169-Cell Differentiation, pubmed-meshheading:7523169-Cell Division, pubmed-meshheading:7523169-Cells, Cultured, pubmed-meshheading:7523169-Female, pubmed-meshheading:7523169-Granulocyte Colony-Stimulating Factor, pubmed-meshheading:7523169-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:7523169-Hematopoietic Cell Growth Factors, pubmed-meshheading:7523169-Hematopoietic Stem Cells, pubmed-meshheading:7523169-Humans, pubmed-meshheading:7523169-Immunophenotyping, pubmed-meshheading:7523169-Interleukin-3, pubmed-meshheading:7523169-Interleukin-6, pubmed-meshheading:7523169-Male, pubmed-meshheading:7523169-Recombinant Proteins, pubmed-meshheading:7523169-Stem Cell Factor, pubmed-meshheading:7523169-Time Factors
pubmed:year
1994
pubmed:articleTitle
Transitional changes in immunophenotypic subpopulations of human peripheral blood CD34+ cells expanded in vitro.
pubmed:affiliation
Department of Internal Medicine II, Hokkaido University School of Medicine, Sapporo, Japan.
pubmed:publicationType
Journal Article