Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1994-10-18
|
| pubmed:abstractText |
Genetically permissive T cell epitopes are an important prerequisite for the development of peptide-based vaccines or immunodiagnostic reagents. We have investigated the structural requirements of permissive T cell recognition of peptide p350-369 from the 38-kDa antigen of Mycobacterium tuberculosis. This peptide was found to be immunogenic in mice of the H-2b, bm12, d, s and k, but not of the H-2f genotype. T cell responses were restricted by I-A class II molecules. The same epitope core was recognized in the H-2b, d and k genotypes. T cell hybrids from BALB/c and C57BL/10 mice were used to determine: (i) the critical residues using substituted peptide derivatives and (ii) the degree of T cell promiscuity. Two out of five BALB/c (H-2d)-derived hybridomas tested displayed promiscuous peptide recognition in the context of H-2b and H-2bm12 antigen-presenting cells. The recognition of critical residues was found to be uniform for all five hybridomas when tested with syngeneic antigen-presenting cells; additional critical residues were identified when the peptide was recognized in the context of allogeneic antigen-presenting cells. Only one of the four tested C57BL/10 (H-2b) hybridomas showed promiscuity in the context of H-2bm12. Each of these C57BL/10-derived clones had a distinct response profile toward the critical residues. We propose that the demonstrated T cell promiscuity involves peptide interaction with polymorphic H-2 I-A residues.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0014-2980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
24
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2061-7
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7522158-Amino Acid Sequence,
pubmed-meshheading:7522158-Animals,
pubmed-meshheading:7522158-Antigen Presentation,
pubmed-meshheading:7522158-Antigens, Bacterial,
pubmed-meshheading:7522158-Epitopes,
pubmed-meshheading:7522158-Female,
pubmed-meshheading:7522158-Haplotypes,
pubmed-meshheading:7522158-Histocompatibility Antigens Class II,
pubmed-meshheading:7522158-Male,
pubmed-meshheading:7522158-Mice,
pubmed-meshheading:7522158-Mice, Inbred Strains,
pubmed-meshheading:7522158-Molecular Sequence Data,
pubmed-meshheading:7522158-Mycobacterium tuberculosis,
pubmed-meshheading:7522158-T-Lymphocytes
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Promiscuous T cell recognition of an H-2 IA-presented mycobacterial epitope.
|
| pubmed:affiliation |
MRC Clinical Sciences Centre, Tuberculosis and Related Infections Unit, Hammersmith Hospital, London, GB.
|
| pubmed:publicationType |
Journal Article
|