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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1994-10-11
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pubmed:abstractText |
P-selectin (GMP-140 or PADGEM) is translocated to the plasma membrane of platelets after platelet activation. P-selectin, therefore, may be a potential marker for evaluating platelet activation. A fluorescence-conjugated immunobinding assay (FCIBA) has been developed to detect specifically P-selectin on platelets. Platelets were isolated from fresh blood by centrifugation and stimulated with various doses of ADP before being fixed with 1% of paraformaldehyde. Fixed platelets were incubated with fluorescence-conjugated anti-P-selectin monoclonal antibody in the wells of fluoricon microtiter plates, and the fluorescence intensity was read on a fluorescence concentration analyzer. Once platelets were fixed, the procedures were completed in < 2 hours. The intra-assay coefficient of variation (CV) was 6.97% (n = 40), the time-based interassay CV was 8.11% (n = 16), and the sample-based inter-assay CV was 6.17% (n = 16). The FCIBA had an excellent correlation (r = 0.936, p < 0.001) with flow cytometry in the measurement of expressed P-selectin in platelets of 20 normal donors. Translocation of P-selectin in plasma-suspended platelets in response to increasing doses of adenosine diphosphate (ADP) occurred in a dose-dependent manner and correlated positively with ADP-induced platelet aggregation in terms of both stimulating doses of ADP (r = 0.99, p < 0.01) and time intervals (r = 0.92, p < 0.05). The findings show that FCIBA is a fast and convenient assay with good precision for the determination of P-selectin expression of human platelets.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Diphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/P-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Membrane Glycoproteins
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-2143
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
124
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
447-54
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7521896-Adenosine Diphosphate,
pubmed-meshheading:7521896-Adult,
pubmed-meshheading:7521896-Antibodies, Monoclonal,
pubmed-meshheading:7521896-Blood Platelets,
pubmed-meshheading:7521896-Cell Adhesion Molecules,
pubmed-meshheading:7521896-Dose-Response Relationship, Drug,
pubmed-meshheading:7521896-Female,
pubmed-meshheading:7521896-Flow Cytometry,
pubmed-meshheading:7521896-Fluorescent Antibody Technique,
pubmed-meshheading:7521896-Humans,
pubmed-meshheading:7521896-Male,
pubmed-meshheading:7521896-P-Selectin,
pubmed-meshheading:7521896-Platelet Activation,
pubmed-meshheading:7521896-Platelet Aggregation,
pubmed-meshheading:7521896-Platelet Membrane Glycoproteins,
pubmed-meshheading:7521896-Time Factors
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pubmed:year |
1994
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pubmed:articleTitle |
A fluorescence-conjugated immunobinding assay for the detection of P-selectin on platelets.
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pubmed:affiliation |
Department of Endocrinology, Mayo Clinic, Rochester, Minnesota.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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