Linked Life Data

7521692

Source:http://linkedlifedata.com/resource/pubmed/id/7521692

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1994-10-12
pubmed:abstractText
We investigated the expression of CD4 antigen in normal bone marrow (BM) samples, enriched in CD34+ hematopoietic progenitor cells. At flow cytometry, a significant fraction (ranging from 25% to 65%) of CD34+ cells also showed low levels of CD4 antigen on their surface. The CD4 receptor densities on the surface of hematopoietic progenitors was approximately 50% that of peripheral blood monocytes and 5% of peripheral blood T lymphocytes. In immunoprecipitation experiments, the CD4 antigen expressed by BM hematopoietic progenitors appeared to be the same form expressed by mature peripheral blood CD4+ cells and appeared to be a potentially functional receptor for human immunodeficiency virus-type 1, because it specifically bound recombinant envelope gp120. Moreover, BM samples, highly enriched in CD34+ cells, showed the presence of CD4 mRNA at reverse transcription-polymerase chain reaction examination. In experiments of complement-mediated cytotoxicity with Leu3-a+Leu3b anti-CD4 monoclonal antibody, a significant reduction in the number of both classes of megakaryocyte (burst-forming unit-meg [BFU-meg] and colony-forming unit-meg [CFU-meg]) and granulocyte/macrophage (CFU-GM) progenitors was observed, whereas erythroid (BFU-E) progenitors were only slightly affected. Moreover, purified CD4+ BM cells obtained by immunomagnetic selection, using high concentrations of Leu3a+Leu3b, showed a colony-forming ability of megakaryocyte and granulocyte/macrophage progenitors comparable with that of CD4- BM cells. In conclusion, the present data show that immature hematopoietic progenitor cells express low levels of CD4, the high-affinity receptor of human immunodeficiency virus-type 1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1896-905
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7521692-Antibodies, Monoclonal, pubmed-meshheading:7521692-Antigens, CD, pubmed-meshheading:7521692-Antigens, CD34, pubmed-meshheading:7521692-Antigens, CD4, pubmed-meshheading:7521692-Base Sequence, pubmed-meshheading:7521692-Bone Marrow Cells, pubmed-meshheading:7521692-Complement System Proteins, pubmed-meshheading:7521692-Cytotoxicity, Immunologic, pubmed-meshheading:7521692-Flow Cytometry, pubmed-meshheading:7521692-Fluorescent Antibody Technique, pubmed-meshheading:7521692-Granulocytes, pubmed-meshheading:7521692-HIV Envelope Protein gp120, pubmed-meshheading:7521692-Hematopoietic Stem Cells, pubmed-meshheading:7521692-Humans, pubmed-meshheading:7521692-Macrophages, pubmed-meshheading:7521692-Megakaryocytes, pubmed-meshheading:7521692-Molecular Sequence Data, pubmed-meshheading:7521692-Polymerase Chain Reaction, pubmed-meshheading:7521692-RNA, Messenger, pubmed-meshheading:7521692-RNA-Directed DNA Polymerase, pubmed-meshheading:7521692-Recombinant Proteins
pubmed:year
1994
pubmed:articleTitle
A subset of human CD34+ hematopoietic progenitors express low levels of CD4, the high-affinity receptor for human immunodeficiency virus-type 1.
pubmed:affiliation
Institute of Human Anatomy, University of Ferrara, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't