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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1994-9-28
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pubmed:abstractText |
Several studies have previously demonstrated enrichment in primitive progenitor cells in subfractions of CD34+ bone marrow (BM) cells not expressing CD38 or HLA-DR (DR) antigens. However, no studies have directly compared these two cell populations with regard to their content of primitive and more committed progenitor cells. Flow cytometric analysis of immunomagnetic isolated CD34+ cells demonstrated little overlap between CD34+CD38- and CD34+DR- progenitor subpopulations in that only 12% to 14% of total CD34+DR- and CD34+CD38- cells were double negative (CD34+CD38-DR-). Although the number of committed myeloid progenitor cells (colony-forming units granulocyte-macrophage) was reduced in both subpopulations, only CD34+CD38- cells were significantly depleted in committed erythroid progenitor cells (burst-forming units-erythroid). In single-cell assay, CD34+CD38- cells showed consistently poorer response to single as opposed to multiple hematopoietic growth factors as compared with unfractionated CD34+ cells, indicating that the CD34+CD38- subset is relatively enriched in primitive hematopoietic progenitor cells. Furthermore, CD34+CD38- and CD34+DR- cells, respectively, formed 3.2-fold and 1.6-fold more high proliferative potential colony-forming cell (HPP-CFC) colonies than did unfractionated CD34+ cells. Finally, CD34+CD38-DR- cells were depleted in HPP-CFCs as compared with CD34+CD38+DR+ cells. The results of the present study suggest that both the CD38- and DR- subfractions of CD34+ bone marrow cells are enriched in primitive hematopoietic progenitor cells, with the CD34+CD38- subpopulation being more highly enriched than CD34+DR- cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADP-ribosyl Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD38,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/CD38 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Erythropoietin,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte Colony-Stimulating...,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1473-81
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7520773-ADP-ribosyl Cyclase,
pubmed-meshheading:7520773-Antigens, CD,
pubmed-meshheading:7520773-Antigens, CD34,
pubmed-meshheading:7520773-Antigens, CD38,
pubmed-meshheading:7520773-Antigens, Differentiation,
pubmed-meshheading:7520773-Bone Marrow,
pubmed-meshheading:7520773-Bone Marrow Cells,
pubmed-meshheading:7520773-Cell Division,
pubmed-meshheading:7520773-Cells, Cultured,
pubmed-meshheading:7520773-Colony-Forming Units Assay,
pubmed-meshheading:7520773-Culture Techniques,
pubmed-meshheading:7520773-Erythropoietin,
pubmed-meshheading:7520773-Flow Cytometry,
pubmed-meshheading:7520773-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:7520773-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:7520773-Growth Substances,
pubmed-meshheading:7520773-HLA-DR Antigens,
pubmed-meshheading:7520773-Hematopoietic Stem Cells,
pubmed-meshheading:7520773-Humans,
pubmed-meshheading:7520773-Interleukin-3,
pubmed-meshheading:7520773-Membrane Glycoproteins,
pubmed-meshheading:7520773-Recombinant Proteins
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pubmed:year |
1994
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pubmed:articleTitle |
Functional differences between CD38- and DR- subfractions of CD34+ bone marrow cells.
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pubmed:affiliation |
Department of Immunology, Norwegian Radium Hospital, Oslo.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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