Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-9-2
pubmed:abstractText
Hyaluronic acid, a major component of the extracellular matrix, plays an important role in the regulation of different cellular processes, e.g., locomotion, cell-cell interaction during morphogenesis, and differentiation. Distribution of hyaluronic acid with respect to the role of sperm hyaluronidase in sperm penetration and gamete interaction is well established. In order to elucidate this mechanism, in our current study we have identified and demonstrated, for the first time, the presence of a 68-kDa cell surface hyaluronic acid binding glycoprotein (HABP) in spermatozoa of different species (rat, mice, bull, and human) by immunoblot analysis and indirect immunofluorescence using the polyclonal antibodies raised against purified HABP. Furthermore, we were able to demonstrate a differential distribution of 68-kDa HA binding protein on the sperm head, midpiece, and tail of different species. To identify its role in sperm function, we observed its declining pattern during epididymal maturation and also the inhibition of sperm-oolemmal adherence by pretreatment of the sperms with anti-HABP antibodies. We have further observed its in vivo phosphorylation in motile spermatozoa. All our data clearly indicate that sperm hyaluronan binding protein may have a specific role in sperm maturation, motility, and fertilization processes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1040-452X
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
69-76
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7519432-Animals, pubmed-meshheading:7519432-Antigens, CD44, pubmed-meshheading:7519432-Carrier Proteins, pubmed-meshheading:7519432-Cattle, pubmed-meshheading:7519432-Cell Membrane, pubmed-meshheading:7519432-Female, pubmed-meshheading:7519432-Fluorescent Antibody Technique, pubmed-meshheading:7519432-Humans, pubmed-meshheading:7519432-Hyaluronic Acid, pubmed-meshheading:7519432-Male, pubmed-meshheading:7519432-Mice, pubmed-meshheading:7519432-Molecular Weight, pubmed-meshheading:7519432-Phosphorylation, pubmed-meshheading:7519432-Rats, pubmed-meshheading:7519432-Rats, Wistar, pubmed-meshheading:7519432-Receptors, Cell Surface, pubmed-meshheading:7519432-Receptors, Lymphocyte Homing, pubmed-meshheading:7519432-Species Specificity, pubmed-meshheading:7519432-Sperm Maturation, pubmed-meshheading:7519432-Sperm Motility, pubmed-meshheading:7519432-Sperm-Ovum Interactions, pubmed-meshheading:7519432-Spermatozoa
pubmed:year
1994
pubmed:articleTitle
Evidence for presence of hyaluronan binding protein on spermatozoa and its possible involvement in sperm function.
pubmed:affiliation
Biochemistry Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, Non-U.S. Gov't