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rdf:type |
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lifeskim:mentions |
umls-concept:C0007600,
umls-concept:C0021757,
umls-concept:C0023462,
umls-concept:C0079460,
umls-concept:C0085424,
umls-concept:C0086418,
umls-concept:C0143630,
umls-concept:C0449432,
umls-concept:C0812215,
umls-concept:C0871261,
umls-concept:C1179435,
umls-concept:C1524073,
umls-concept:C1548799,
umls-concept:C1704632,
umls-concept:C1705248,
umls-concept:C1706586,
umls-concept:C1706817,
umls-concept:C2746015,
umls-concept:C2911692
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pubmed:issue |
4
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pubmed:dateCreated |
1994-8-31
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pubmed:abstractText |
We have examined the role of Raf-1 in the mitogenic response of the factor-deprived human megakaryoblastic leukemia cell line MO7 to recombinant human granulocyte-macrophage colony-stimulating factor, interleukin 3, interleukin 9, and stem cell factor by using c-raf antisense oligodeoxyribonucleotides. Uptake of oligodeoxyribonucleotides by MO7 cells was maximal at 5-10 h in culture, and oligomers remained stable in these cells for at least 24 h. Treatment of MO7 cells with the antisense oligomer resulted in intracellular oligomer/mRNA duplex formation followed by efficient translation blockade of c-raf-1. In contrast, sense and non-sense oligodeoxyribonucleotides failed to form intracellular duplexes and did not interfere with translation of c-raf-1, suggesting specific elimination of c-raf-1 by the antisense oligodeoxyribonucleotide. Furthermore, exposure of MO7 cells to c-raf-1 antisense prevented factor-induced nuclear translocation of Raf-1. Most importantly, proliferation of MO7 cells ([3H]thymidine incorporation) enabled by these growth factors was significantly reduced when the c-raf-1 antisense oligodeoxyribonucleotide was added to cultures, whereas the mitogenic response to these factors remained almost unaffected in the presence of sense and non-sense oligodeoxyribonucleotides.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Codon,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Hematopoietic Cell Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-9,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogens,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-raf,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Stem Cell Factor
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1044-9523
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
367-72
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7519043-Base Sequence,
pubmed-meshheading:7519043-Cell Division,
pubmed-meshheading:7519043-Codon,
pubmed-meshheading:7519043-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:7519043-Growth Substances,
pubmed-meshheading:7519043-Hematopoietic Cell Growth Factors,
pubmed-meshheading:7519043-Humans,
pubmed-meshheading:7519043-Interleukin-3,
pubmed-meshheading:7519043-Interleukin-9,
pubmed-meshheading:7519043-Leukemia, Megakaryoblastic, Acute,
pubmed-meshheading:7519043-Mitogens,
pubmed-meshheading:7519043-Molecular Sequence Data,
pubmed-meshheading:7519043-Oligonucleotides, Antisense,
pubmed-meshheading:7519043-Protein-Serine-Threonine Kinases,
pubmed-meshheading:7519043-Proto-Oncogene Proteins,
pubmed-meshheading:7519043-Proto-Oncogene Proteins c-raf,
pubmed-meshheading:7519043-Recombinant Proteins,
pubmed-meshheading:7519043-Stem Cell Factor,
pubmed-meshheading:7519043-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
Raf-1 is a necessary component of the mitogenic response of the human megakaryoblastic leukemia cell line MO7 to human stem cell factor, granulocyte-macrophage colony-stimulating factor, interleukin 3, and interleukin 9.
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pubmed:affiliation |
Department of Medical Oncology and Applied Molecular Biology, Free University of Berlin, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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