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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1994-8-22
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pubmed:abstractText |
alpha-(Methylamino)isobutyric acid (MeAIB) insensitive Na(+)-dependent alanine transport activity in the bovine kidney cell line NBL-1 was increased upon amino acid starvation (> or = 20% over control levels). When L-phenylalanine (3 mM) was included in the starvation medium the increase was further enhanced (> or = 85% over control levels). In cells grown in control medium the Vmax, for MeAIB-insensitive Na+/alanine co-transport was found to be 6.0 +/- 0.7 nmol/3 min per mg (Km 41 +/- 12 microM) and for L-phenylalanine-treated amino-acid-starved cells the Vmax. was 21 +/- 5 nmol/3 min per mg (Km 92 +/- 40 microM). The increase in Vmax. was prevented by cycloheximide. Substrate specificity analysis identified the L-phenylalanine-induced transport system as System B0. [35S]Methionine labelling of cells during the amino acid starvation/phenylalanine treatments resulted in the differential labelling of a protein of 78 kDa. Northern-blot analysis using a SAAT1-specific probe revealed the presence of a new transcript (3.2 kb) in RNA extracted from cells incubated in amino acid starvation medium with L-phenylalanine included. The present findings suggest a novel means of control for System B0 by the use of physiological stress. It is also proposed that SAAT1 and System-B0 transcripts have considerable sequence similarity.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-1280334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-1372904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-1429548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-1550853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-1599439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-1731772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-1740408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-2490366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-2557766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-3045517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-3580363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-569556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-6200477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-6303424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-6901532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-8240287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-8343134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-8420925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-8430828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-8435065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-8484724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7519009-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2,2-dimethyl-beta-alanine,
http://linkedlifedata.com/resource/pubmed/chemical/Alanine,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport Systems, Basic,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport Systems...,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Methionine,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylalanine,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Alanine
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
301 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
399-405
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pubmed:dateRevised |
2010-8-25
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pubmed:meshHeading |
pubmed-meshheading:7519009-Alanine,
pubmed-meshheading:7519009-Amino Acid Transport Systems, Basic,
pubmed-meshheading:7519009-Amino Acid Transport Systems, Neutral,
pubmed-meshheading:7519009-Amino Acids,
pubmed-meshheading:7519009-Animals,
pubmed-meshheading:7519009-Biological Transport,
pubmed-meshheading:7519009-Blotting, Northern,
pubmed-meshheading:7519009-Carrier Proteins,
pubmed-meshheading:7519009-Cattle,
pubmed-meshheading:7519009-Cell Line,
pubmed-meshheading:7519009-Culture Media,
pubmed-meshheading:7519009-DNA Probes,
pubmed-meshheading:7519009-Epithelium,
pubmed-meshheading:7519009-Kidney,
pubmed-meshheading:7519009-Kinetics,
pubmed-meshheading:7519009-Methionine,
pubmed-meshheading:7519009-Phenylalanine,
pubmed-meshheading:7519009-RNA,
pubmed-meshheading:7519009-Sodium,
pubmed-meshheading:7519009-beta-Alanine
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pubmed:year |
1994
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pubmed:articleTitle |
Regulation of System B0 amino-acid-transport activity in the renal epithelial cell line NBL-1 and concomitant changes in SAAT1 hybridizing transcripts.
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pubmed:affiliation |
Department of Biochemistry, University of Bristol, School of Medical Sciences, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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