Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1994-8-24
pubmed:abstractText
Infection of humans with verotoxin-producing Escherichia coli (VTEC) O113:H21 is associated with clinical features comparable to those associated with infection with attaching and effacing VTEC strains including those of serotype O157:H7. We have shown previously that the adhesion phenotype of VTEC O157:H7 is influenced by the presence of a homolog of the chromosomal eaeA (for E. coli attaching and effacing) gene. In contrast, by colony blot hybridization, VTEC O113:H21 is negative for the eaeA gene. Therefore, the aim of this study was to define the adhesion phenotype of VTEC O113:H21 strain CL-15 to both cultured epithelial cells (HEp-2) and rabbit intestine in vivo. Under transmission electron microscopy, areas of microvillus effacement were observed in regions directly beneath the organism in CL-15-infected cells both in vitro and in vivo. However, F-actin adhesion pedestals on the host plasma membrane were absent. Failure of CL-15 to induce polymerization of actin was confirmed by using staining of F-actin with fluorescein-labeled phalloidin. Under indirect immunofluorescence microscopy, CL-15-infected HEp-2 cells also failed to demonstrate the recruitment of another cytoskeletal element, alpha-actinin, below foci of bacterial adhesion. In contrast, VTEC O157:H7 infection of HEp-2 cells was associated with increased alpha-actinin immunofluorescence. These findings suggest that bacterial factors distinct from those of EaeA are necessary for the adhesion phenotype of VTEC O113:H21.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-1346880, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-1396556, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-1398907, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-1433251, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-1552854, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-1577255, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-1587620, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-1630295, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-1671778, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-1969849, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-2004829, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-2019431, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-2172966, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-2407656, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-2498392, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-2644022, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-2647635, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-2667390, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-2886431, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-291981, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-3010813, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-3278980, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-3316033, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-3543152, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-3886804, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-6350186, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-7014727, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-77415, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-8174877, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-8376594, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-8376595, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-8380793, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-8392972, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-8406796, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-8500884, http://linkedlifedata.com/resource/pubmed/commentcorrection/7518809-8514377
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:geneSymbol
eaeA
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3494-505
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Distinct binding properties of eaeA-negative verocytotoxin-producing Escherichia coli of serotype O113:H21.
pubmed:affiliation
Division of Gastroenterology, Hospital for Sick Children, Toronto, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't