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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1994-8-23
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pubmed:abstractText |
We have examined the range of mucosal and systemic immune responses induced by oral or parenteral immunization with ovalbumin (OVA) entrapped in poly(D,L-lactide-co-glycolide) (PLG) microparticles. A single subcutaneous immunization with OVA-PLG primed significant OVA-specific IgG and delayed-type hypersensitivity (DTH) responses. The DTH responses were of similar magnitude to those obtained using immunostimulating complexes (ISCOMS) as a potent control adjuvant, although ISCOMS stimulated higher serum IgG responses. Both vectors also primed OVA-specific in vitro proliferative responses in draining lymph node cells following a single immunization and strong OVA-specific CTL responses were found after intraperitoneal (i.p.) immunization. ISCOMS were more efficient in inducing cytotoxic T lymphocytes (CTL), requiring much less antigen and only ISCOMS could stimulate primary OVA-specific CTL responses in the draining lymph nodes. Multiple oral immunizations with OVA in PLG microparticles or in ISCOMS resulted in OVA-specific CTL responses and again ISCOMS seemed more potent as fewer feeds were necessary. Lastly, multiple feeds of OVA in PLG microparticles generated significant OVA-specific intestinal IgA responses. This is the first demonstration that PLG microparticles can stimulate CTL responses in vivo and our results highlight their ability to prime a variety of systemic and mucosal immune responses which may be useful in future oral vaccine development.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-1323901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-1538138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-1587538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-1588035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-1613454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-1786069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-1833459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-1879922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-2026440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-2071168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-2184369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-2293220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-2586628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-2812009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-3261634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-7508416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-7678924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-7679422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-8104409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-8104882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-8212845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-8419476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-8438613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518802-8464808
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
661-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7518802-Adjuvants, Immunologic,
pubmed-meshheading:7518802-Administration, Oral,
pubmed-meshheading:7518802-Animals,
pubmed-meshheading:7518802-Cell Division,
pubmed-meshheading:7518802-Epitopes,
pubmed-meshheading:7518802-Female,
pubmed-meshheading:7518802-Hypersensitivity, Delayed,
pubmed-meshheading:7518802-ISCOMs,
pubmed-meshheading:7518802-Immunization,
pubmed-meshheading:7518802-Immunoglobulin G,
pubmed-meshheading:7518802-Injections, Subcutaneous,
pubmed-meshheading:7518802-Lactic Acid,
pubmed-meshheading:7518802-Mice,
pubmed-meshheading:7518802-Mice, Inbred BALB C,
pubmed-meshheading:7518802-Mice, Inbred C57BL,
pubmed-meshheading:7518802-Ovalbumin,
pubmed-meshheading:7518802-Polyglycolic Acid,
pubmed-meshheading:7518802-Polymers,
pubmed-meshheading:7518802-T-Lymphocytes,
pubmed-meshheading:7518802-T-Lymphocytes, Cytotoxic
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pubmed:year |
1994
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pubmed:articleTitle |
Induction of mucosal and systemic immune responses by immunization with ovalbumin entrapped in poly(lactide-co-glycolide) microparticles.
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pubmed:affiliation |
Department of Immunology, University of Glasgow, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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