Linked Life Data

7518450

Source:http://linkedlifedata.com/resource/pubmed/id/7518450

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
29
pubmed:dateCreated
1994-8-15
pubmed:abstractText
We have previously isolated detergent-resistant membrane complexes from lysates of cultured kidney epithelial cells. These structures are enriched in proteins anchored by glycosylphosphatidylinositol, which are localized to the apical plasma membrane of epithelial cells, and have a distinctive lipid composition. We now report that the membranes contain p62yes, a non-receptor tyrosine kinase of the Src family. p62yes is the only major tyrosine-phosphorylated protein in the membrane complexes when they are isolated. Several proteins in the complexes can serve as substrates for phosphorylation by p62yes in vitro. The kinase is enriched at least 10-fold in the insoluble membranes compared to cellular membranes. p60c-src and p60v-src, which are highly homologous to p62yes, are not present in the complexes, even when the membranes are isolated from cells that overexpress the proteins. As p62yes is located near the apical plasma membrane of epithelial cells in several tissues in vivo, interaction of the kinase with other components of a detergent-resistant membrane microdomain may play an important role in transmembrane signal transduction.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
269
pubmed:geneSymbol
src, yes
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19123-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Triton X-100-resistant membrane complexes from cultured kidney epithelial cells contain the Src family protein tyrosine kinase p62yes.
pubmed:affiliation
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794-5215.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't