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rdf:type |
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lifeskim:mentions |
umls-concept:C0026809,
umls-concept:C0030956,
umls-concept:C0037081,
umls-concept:C0038952,
umls-concept:C0085358,
umls-concept:C0205369,
umls-concept:C0441587,
umls-concept:C0547040,
umls-concept:C0871261,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1704632,
umls-concept:C1706438,
umls-concept:C1706817,
umls-concept:C1708111,
umls-concept:C2698600,
umls-concept:C2911692
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pubmed:issue |
15
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pubmed:dateCreated |
1994-8-15
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pubmed:abstractText |
CD8+ T-lymphocytes (TCD8+) recognize minimal peptides of 8-10 residues which are the products of intracellularly processed proteins and are presented at the cell surface by major histocompatibility complex class I molecules. An important step in this process is the translocation of processed proteins from the cytosol across the endoplasmic reticulum membrane, mediated by transporter associated with antigen-processing proteins or alternatively by endoplasmic reticulum-insertion signal sequences located at the NH2-terminus of the precursor molecules. We report here that the addition of an endoplasmic reticulum-insertion signal sequence at the NH2-terminus of TCD8+ epitopes from chicken ovalbumin (amino acids 257-264) or a naturally occurring tumor antigen expressed by the murine mastocytoma P815 (P1A amino acids 35-43) significantly enhanced the priming of specific TCD8+ in vivo. The signal sequence did not enhance peptide immunogenicity by merely increasing the hydrophobicity of the peptide, since ovalbumin amino acids 257-264 peptide with the signal sequence at its COOH-terminus did not demonstrate enhanced efficacy. The signal sequence did not act as a helper epitope, since TCD8+ responses were not diminished in class II-deficient transgenic mice or in mice depleted of CD4+ T-cells in vivo. Importantly, a single immunization with the fusion peptide significantly prolonged survival of mice challenged with E.G7OVA, a thymoma transfected with the complementary DNA of chicken ovalbumin.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-1375265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-1381312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-1384958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-1532538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-1538752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-1682377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-1684372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-1692084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-1833459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-1853786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-1856631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-1903428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-1946358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-1992491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-2433584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-2452183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-2469442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-2469721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-2521235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-2568889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-2586628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-2784569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-2834454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-2955227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-2966865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-2972241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-3261634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-6992981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-7679422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-8095512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-8170938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-8242733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-8283027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-8283036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-8325345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-8340755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7518351-8381832
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0008-5472
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4155-61
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pubmed:dateRevised |
2011-9-15
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pubmed:meshHeading |
pubmed-meshheading:7518351-Animals,
pubmed-meshheading:7518351-Mice,
pubmed-meshheading:7518351-Ovalbumin,
pubmed-meshheading:7518351-Thymus Neoplasms,
pubmed-meshheading:7518351-Female,
pubmed-meshheading:7518351-Thymoma,
pubmed-meshheading:7518351-Tumor Cells, Cultured,
pubmed-meshheading:7518351-Epitopes,
pubmed-meshheading:7518351-Endoplasmic Reticulum,
pubmed-meshheading:7518351-Amino Acid Sequence,
pubmed-meshheading:7518351-Immunotherapy,
pubmed-meshheading:7518351-Lymphocyte Activation,
pubmed-meshheading:7518351-Molecular Sequence Data,
pubmed-meshheading:7518351-Mice, Inbred C57BL,
pubmed-meshheading:7518351-Mice, Inbred DBA,
pubmed-meshheading:7518351-Signal Transduction,
pubmed-meshheading:7518351-T-Lymphocyte Subsets,
pubmed-meshheading:7518351-Transfection,
pubmed-meshheading:7518351-Recombinant Fusion Proteins
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