Linked Life Data

7517074

Source:http://linkedlifedata.com/resource/pubmed/id/7517074

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1994-7-26
pubmed:abstractText
The effect of platelet procoagulant activity in the Activated Coagulation Time (ACT) was measured in whole blood anticoagulated with various levels of heparin before or after reversal with protamine. Similar studies were carried out on blood anticoagulated with hirudin to distinguish procoagulant activity from heparin neutralization in platelet preparations. At 0.5-1.0 units/mL antithrombin activity with heparin or hirudin, the ACT was lowered progressively by the addition of increasing concentrations of lysed platelets to as much as 20 seconds below the baseline clotting time obtained with unanticoagulated blood samples. Neutralization of higher concentrations of heparin with protamine produced an ACT below baseline in the presence of lysed platelets. Aprotinin (400 KIU/mL) prolonged the ACT slightly in heparinized whole blood, but did not prevent the lowering of the ACT by lysed platelets to baseline or below. Recirculation of heparinized whole blood in a simulated cardiopulmonary bypass circuit generated platelet microparticles detected by flow cytometry. An increase in platelet microparticles was associated with a decrease in the amount of protamine needed to reach the baseline ACT in blood samples removed from the circuit at various time points during recirculation. A chromogenic anti-Factor Xa assay of heparin did not show a change with increasing microparticle concentration during recirculation. These findings indicate a masking of heparin activity by the procoagulant activity of platelet membrane microparticles that could affect reversal of heparin based on the ACT.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0049-3848
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
285-300
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Masking of heparin activity in the activated coagulation time (ACT) by platelet procoagulant activity.
pubmed:affiliation
Department of Pathology and Laboratory Medicine, East Carolina University School of Medicine, Greenville 27858.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't