Linked Life Data

7516490

Source:http://linkedlifedata.com/resource/pubmed/id/7516490

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-7-20
pubmed:abstractText
A fusion between the recA gene of Rhizobium phaseoli and the lacZ gene was constructed in vitro and cloned in a mini-Tn5 transposon derivative to obtain chromosomal insertions which make it possible to quantitatively examine their transcriptional regulation in both R. phaseoli and E. coli. Likewise, and by insertion of a spectinomycin-resistance gene cassette into the recA gene of R. phaseoli and subsequent marker exchange, a RecA- derivative of this bacterial species has been obtained. Analysis of this recA-lacZ fusion showed that it was inducible by DNA damage in the RecA+ strain of R. phaseoli but not in the RecA- mutant. On the other hand, the recA-lacZ fusion of R. phaseoli was not induced in DNA-damaged RecA+ cells of E. coli. Furthermore, the range of UV doses which give rise to dose dependence in the induction of its respective recA genes is different in R. phaseoli from that in E. coli.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
308
pubmed:geneSymbol
lacZ, recA
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
99-107
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Autoregulation and kinetics of induction of the Rhizobium phaseoli recA gene.
pubmed:affiliation
Department of Genetics and Microbiology, Faculty of Sciences, Autonomous University of Barcelona, Bellaterra, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't