Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1994-7-18
pubmed:abstractText
GRB2, a 25-kDa protein comprising a single SH2 domain flanked by two SH3 domains, has been implicated in linking receptor protein tyrosine kinases (PTKs) to the Ras pathway by interacting with the guanine nucleotide exchange protein SOS. Previous studies have demonstrated that GRB2 directly interacts with Shc, a proto-oncogene product that is tyrosine phosphorylated upon receptor and nonreceptor PTK activation. In this report, we detected low levels of tyrosine phosphorylation of Shc and induced association with GRB2 upon T-cell receptor (TCR) stimulation. Instead, a prominent 36- to 38-kDa tyrosine phosphoprotein (pp36-38) associated with the SH2 domain of GRB2 and formed a stable complex with GRB2/SOS upon TCR stimulation. Cellular fractionation studies showed that whereas both GRB2 and SOS partitioned to the soluble and particulate fractions, pp36-38 was present exclusively in the particulate fraction. This phosphoprotein had the same apparent mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis as the phosphoprotein that associates with phospholipase C-gamma 1 (PLC-gamma 1). Furthermore, following partial immunodepletion of GRB2 and of the associated pp36-38, there was a significant reduction in the amount of the 36-kDa phosphoprotein associated with PLC-gamma 1, suggesting that a trimeric PLC-gamma 1/pp36-38/GRB2 complex could form. In support of this notion, we have also been able to detect low levels of PLC-gamma 1 in GRB2 immunoprecipitates. We suggest that pp36-38 may be a bridging protein, coupling different signalling molecules to cytoplasmic PTKs regulated by the TCR.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1280326, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1311054, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1320023, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1322798, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1372395, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1400471, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1409579, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1418701, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1423621, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1425589, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1465135, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1472473, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1500851, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1505025, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1526965, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1549349, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1604323, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1620132, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1623525, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1671341, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1689750, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1700866, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1706070, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1708307, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1712101, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1828897, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-1934068, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-2015626, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-2061301, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-2122974, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-2123303, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-2138816, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-2190221, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-2198577, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-2201921, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-2217205, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-2257629, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-2374928, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-2450282, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-2786526, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-6208306, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-7510700, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-7688728, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8236435, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8316835, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8334708, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8346442, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8366117, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8386805, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8417322, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8441421, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8449939, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8462097, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8462098, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8479536, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8479540, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8479541, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8484124, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8490966, http://linkedlifedata.com/resource/pubmed/commentcorrection/7516467-8493579
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing, http://linkedlifedata.com/resource/pubmed/chemical/GRB2 Adaptor Protein, http://linkedlifedata.com/resource/pubmed/chemical/GRB2 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase, http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine, http://linkedlifedata.com/resource/pubmed/chemical/Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor, http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases, http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
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