Linked Life Data

7516455

Source:http://linkedlifedata.com/resource/pubmed/id/7516455

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-7-21
pubmed:abstractText
The mode(s) of skeletal muscle protein turnover as well as muscle and animal growth may be studied by using lean and obese animals as models. The objectives of this study were to look at proteolytic variables implicated in these processes. A lean and obese strain of swine from similar genetic lineage (Duroc x Yorkshire, 50:50) have been well established and may prove ideal for this purpose. This study was done in two phases. Phase I included eight lean and eight obese pigs at 2.5 months of age, and phase II was identical, but the pigs were 7 months old. Longissimus muscle samples were processed immediately after euthanasia for activity measurements of mu-calpain, m-calpain, calpastatin, and lysosomal cathepsins B and B + L. Additional samples were taken for DNA, RNA, and total protein determinations. In phase I, total calpastatin activity, total and specific cathepsin B+L activity, and total protein/g muscle were greater in the obese pigs than in the lean pigs. In contrast, DNA and RNA/g muscle were greater in the lean pigs. No other differences were observed in phase I. In phase II, total calpastatin activity and total cathepsin B activity were greater in the obese pigs than in the lean pigs. No other differences were observed in phase II. From phase I to phase II, mu-calpain total activity increased in the lean pigs but not in the obese pigs and calpastatin activity decreased in both lean and obese pigs; however, the phase-II-obese and phase-I-lean total calpastatin concentrations were not significantly different.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0023-6764
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
38-41
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Comparison of proteolytic variables in a lean and obese strain of pig at the ages of 2.5 and 7 months.
pubmed:affiliation
U.S. Department of Agriculture, ARS, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, NE 68933.
pubmed:publicationType
Journal Article, Comparative Study