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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1994-7-20
|
| pubmed:abstractText |
Previous work demonstrated that pretreatment of mice with low doses of the organochlorine insecticide chlordecone (CD) altered the tissue disposition of a subsequent [14C]CD or [14C]cholesterol challenge dose. The profile of these changes was consistent with the induction of a protein integral to hepatic CD/cholesterol turnover. The present study was undertaken to confirm similar in vivo effects in the rat and to analyze potential CD-induced changes in hepatic transport kinetics in the perfused rat liver. For in vivo experiments, male, Sprague-Dawley rats were treated with CD (5, 15, or 40 mg/kg) and challenged 3 or 7 days later with a 5 mg/kg [14C]CD tracer dose. Rats challenged 3 days after treatment and evaluated 16 hr later showed a dose-dependent decrease in hepatic [14C]CD relative to controls. This decrease could not be attributed to alterations in liver mass or total liver lipid. For kinetics studies, rats received 15 mg/kg CD and livers were perfused 3 days later. Following a brief (5-7 min) single-pass perfusion, the perfusate was replaced with recirculating buffer containing albumin-bound [3H]oleic acid or high-density lipoprotein-bound [14C]CD or [14C]cholesterol. Livers from pretreated animals had significantly decreased rates of [14C]CD and [14C]cholesterol uptake. Efflux of [14C]CD and biliary excretion of [14C]cholesterol were increased. No changes were observed in uptake or biliary excretion of [3H]oleic acid. SDS-PAGE of hepatic cytosol revealed an enhanced band intensity corresponding to a M(r) of 25,600 in livers from pretreated rats.(ABSTRACT TRUNCATED AT 250 WORDS)
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/7-Alkoxycoumarin O-Dealkylase,
http://linkedlifedata.com/resource/pubmed/chemical/Albumins,
http://linkedlifedata.com/resource/pubmed/chemical/Chlordecone,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 CYP1A1,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oleic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidoreductases
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0272-0590
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
22
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
286-92
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7516299-7-Alkoxycoumarin O-Dealkylase,
pubmed-meshheading:7516299-Albumins,
pubmed-meshheading:7516299-Animals,
pubmed-meshheading:7516299-Bile,
pubmed-meshheading:7516299-Chlordecone,
pubmed-meshheading:7516299-Cholesterol,
pubmed-meshheading:7516299-Cytochrome P-450 CYP1A1,
pubmed-meshheading:7516299-Cytochrome P-450 Enzyme System,
pubmed-meshheading:7516299-Cytosol,
pubmed-meshheading:7516299-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7516299-Enzyme Induction,
pubmed-meshheading:7516299-Lipid Metabolism,
pubmed-meshheading:7516299-Lipoproteins,
pubmed-meshheading:7516299-Liver,
pubmed-meshheading:7516299-Male,
pubmed-meshheading:7516299-Oleic Acids,
pubmed-meshheading:7516299-Organ Size,
pubmed-meshheading:7516299-Oxidoreductases,
pubmed-meshheading:7516299-Rats,
pubmed-meshheading:7516299-Rats, Sprague-Dawley,
pubmed-meshheading:7516299-Tissue Distribution
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Chlordecone pretreatment alters [14C]chlordecone and [14C]cholesterol transport kinetics in the perfused rat liver.
|
| pubmed:affiliation |
Department of Fisheries and Wildlife, Oregon State University, Corvallis 97331.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
|