Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1994-7-12
|
| pubmed:abstractText |
The Wsh is a mutant allele at the W (c-kit) locus of mice, but no significant abnormalities are found at the coding region of the Wsh allele. Since cultured mast cells derived from the spleen of Wsh/Wsh mice do not express messenger RNA (mRNA) of c-kit, we studied the interrelation between the number of mast cells and the magnitude of c-kit mRNA expression in the skin of Wsh/Wsh mice of various ages. The number of mast cells in the skin of Wsh/Wsh embryos of 18 days postcoitum (pc) was approximately 40% that of normal control (+/+) embryos, but the number of mast cells decreased exponentially after birth; the number dropped to 0.6% that of +/+ mice at day 150 after birth. A weak but apparent signal of c-kit mRNA was detectable in the skin of 18-day pc Wsh/Wsh embryos by RNase protection assay but not in the skin of 5-day-old Wsh/Wsh mice. The number of c-kit protein-containing cells was significantly greater in the skin of 18-day pc Wsh/Wsh embryos than in the skin of 5-day-old Wsh/Wsh mice. The abolishment of c-kit mRNA expression appeared to be specific, because the expression of mast cell carboxypeptidase A mRNA but not of c-kit mRNA was detectable by in situ hybridization in skin mast cells of 5-day-old Wsh/Wsh mice. Taken together, the expression of c-kit mRNA was abolished first, then the content of c-kit protein dropped to undetectable levels, and then the disappearance of Wsh/Wsh mast cells themselves followed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-kit,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Colony-Stimulating Factor
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
83
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3509-16
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:7515715-Age Factors,
pubmed-meshheading:7515715-Animals,
pubmed-meshheading:7515715-Animals, Newborn,
pubmed-meshheading:7515715-Base Sequence,
pubmed-meshheading:7515715-Female,
pubmed-meshheading:7515715-Gene Expression Regulation,
pubmed-meshheading:7515715-Mast Cells,
pubmed-meshheading:7515715-Mice,
pubmed-meshheading:7515715-Mice, Inbred C57BL,
pubmed-meshheading:7515715-Molecular Sequence Data,
pubmed-meshheading:7515715-Mutation,
pubmed-meshheading:7515715-Pregnancy,
pubmed-meshheading:7515715-Proto-Oncogene Proteins,
pubmed-meshheading:7515715-Proto-Oncogene Proteins c-kit,
pubmed-meshheading:7515715-Proto-Oncogenes,
pubmed-meshheading:7515715-RNA, Messenger,
pubmed-meshheading:7515715-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:7515715-Receptors, Colony-Stimulating Factor,
pubmed-meshheading:7515715-Skin
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
C-kit gene is expressed by skin mast cells in embryos but not in puppies of Wsh/Wsh mice: age-dependent abolishment of c-kit gene expression.
|
| pubmed:affiliation |
Department of Pathology, Osaka University Medical School, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|