Linked Life Data

7515283

Source:http://linkedlifedata.com/resource/pubmed/id/7515283

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1994-7-7
pubmed:abstractText
Through functional studies of mutant tRNAs, we have identified sequence and/or structural features important for specifying the many distinctive properties of E coli initiator tRNA. Many of the mutant tRNAs contain an anticodon sequence change from CAU-->CUA and are now substrates for E coli glutaminyl-tRNA synthetase (GlnRS). We describe here the effect of further mutating the discriminator base 73 and nucleotide 72 at the end of the acceptor stem on: i) recognition of the mutant tRNAs by E coli GlnRS; ii) recognition by E coli methionyl-tRNA transformylase; and iii) activity of the mutant tRNAs in initiation in E coli. For GlnRS recognition, our results are, in general, consistent with interactions found in the crystal structure of the E coli GlnRS-glutamine tRNA complex. The results also support our previous conclusion that formylation of initiator tRNA is important for its function in initiation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0300-9084
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1051-60
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7515283-Acyltransferases, pubmed-meshheading:7515283-Amino Acyl-tRNA Synthetases, pubmed-meshheading:7515283-Base Sequence, pubmed-meshheading:7515283-Binding Sites, pubmed-meshheading:7515283-Escherichia coli, pubmed-meshheading:7515283-Hydroxymethyl and Formyl Transferases, pubmed-meshheading:7515283-Immunoblotting, pubmed-meshheading:7515283-Molecular Sequence Data, pubmed-meshheading:7515283-Mutation, pubmed-meshheading:7515283-Nucleic Acid Conformation, pubmed-meshheading:7515283-Peptide Chain Initiation, Translational, pubmed-meshheading:7515283-RNA, Bacterial, pubmed-meshheading:7515283-RNA, Transfer, Amino Acyl, pubmed-meshheading:7515283-RNA, Transfer, Met, pubmed-meshheading:7515283-Structure-Activity Relationship, pubmed-meshheading:7515283-Substrate Specificity
pubmed:year
1993
pubmed:articleTitle
Relationship between the structure and function of Escherichia coli initiator tRNA.
pubmed:affiliation
Institute of Cell and Molecular Biology, University of Edinburgh, UK.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.