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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1994-6-29
|
| pubmed:abstractText |
We have isolated a histamine release factor from human platelet supernatants by heparin column chromatography, gel filtration and reversed phase HPLC. Fractions from each chromatographic step were assayed for histamine release factor activity (HRF). A peak of HRF activity was detected at a molecular mass of 60 kDa. Subsequent HPLC purification showed that the factor co-eluted with human serum albumin, but was totally extractable into the lipid phase. Comparison of biological activity with known basophil and eosinophil-activating hydroxyeicosanoids demonstrated similar activity with 5(S)-hydroperoxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (5(S)-HPETE). The identification of this lipid soluble HRF demonstrates the novel potential role of an as yet unidentified lipid as an HRF, in the absence of priming stimuli.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
30
|
| pubmed:volume |
201
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
443-8
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7515234-Amino Acid Sequence,
pubmed-meshheading:7515234-Blood Platelets,
pubmed-meshheading:7515234-Histamine Release,
pubmed-meshheading:7515234-Humans,
pubmed-meshheading:7515234-Leukotrienes,
pubmed-meshheading:7515234-Molecular Sequence Data,
pubmed-meshheading:7515234-Peptide Fragments,
pubmed-meshheading:7515234-Serum Albumin
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Isolation of a lipid-soluble histamine release factor from human platelets.
|
| pubmed:affiliation |
Glaxo Institute for Molecular Biology, Geneva, Switzerland.
|
| pubmed:publicationType |
Journal Article
|