Linked Life Data

7513719

Source:http://linkedlifedata.com/resource/pubmed/id/7513719

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-6-6
pubmed:abstractText
The synaptic circuitry of the intrinsic GABAergic system of the central extended amygdala (CEA) in relation to efferent neurons and cortical afferents was examined in the present study. Neurons in the CEA projecting to the dorsal vagal complex and the parabrachial complex were identified by the retrograde transport of wheat germ agglutinin-horseradish peroxidase (WGA-HRP). Postembedding GABA-immunocytochemistry revealed that GABA-immunoreactive (GABA-IR) terminals formed largely symmetrical synaptic contacts with the perikarya and proximal dendritic processes of almost all WGA-HRP-labeled neurons in the CEA. To determine the relationship between cortical afferents and CEA GABAergic neurons, WGA-HRP was used to anterogradely label afferents from the insular cortex in combination with postembedding immunogold detection of GABA. Cortical afferents formed asymmetrical synaptic contacts predominantly on small dendrites and dendritic spines. Many of the dendrites postsynaptic to cortical terminals in the central nucleus were immunoreactive for GABA although only relatively few spines were GABA-IR. Combining pre-embedding GAD-immunocytochemistry with cortical lesions resulted in approximately 40% of degenerating terminals of insular cortical origin in the central nucleus in contact with small, GAD-IR dendrites and spines. The present results demonstrate that the neurons providing the major CEA outputs to the brainstem receive an extensive GABAergic innervation, strongly supporting our proposal that CEA efferent neurons are under strong tonic inhibition by intrinsic GABAergic neurons. Further, our finding that the major cortical input to the central nucleus preferentially innervates intrinsic GABAergic neurons suggests that these neurons in the CEA may serve as an interface between the principal inputs and outputs of this forebrain region.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9967
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
340
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
43-64
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7513719-Amygdala, pubmed-meshheading:7513719-Animals, pubmed-meshheading:7513719-Brain Stem, pubmed-meshheading:7513719-Cerebral Cortex, pubmed-meshheading:7513719-Glutamate Decarboxylase, pubmed-meshheading:7513719-Horseradish Peroxidase, pubmed-meshheading:7513719-Immunohistochemistry, pubmed-meshheading:7513719-Male, pubmed-meshheading:7513719-Microscopy, Electron, pubmed-meshheading:7513719-Nerve Degeneration, pubmed-meshheading:7513719-Nerve Endings, pubmed-meshheading:7513719-Neural Pathways, pubmed-meshheading:7513719-Neurons, Afferent, pubmed-meshheading:7513719-Plastic Embedding, pubmed-meshheading:7513719-Presynaptic Terminals, pubmed-meshheading:7513719-Rats, pubmed-meshheading:7513719-Rats, Sprague-Dawley, pubmed-meshheading:7513719-Synapses, pubmed-meshheading:7513719-Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate, pubmed-meshheading:7513719-Wheat Germ Agglutinins, pubmed-meshheading:7513719-gamma-Aminobutyric Acid
pubmed:year
1994
pubmed:articleTitle
Evidence for a GABAergic interface between cortical afferents and brainstem projection neurons in the rat central extended amygdala.
pubmed:affiliation
Department of Anatomy, University of Iowa, Iowa City 52242.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.