Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1994-6-1
|
| pubmed:abstractText |
We investigated the surface expression of leukocyte differentiation antigens and the Ig heavy-chain variable region (VH) gene family use in leukemic cells from 26 Japanese patients with chronic B-cell leukemias with special reference to CD5 antigen expression. CD5 was expressed on leukemic cells in 21 of 26 cases (CD5+) but not in 5 cases (CD5-). Myelomonocytic marker, CD13 antigen was expressed on the leukemic cells in all 5 CD5- cases but in none of CD5+ cases. Leukemic cells in CD5- cases also expressed CD11b antigen more frequently than those in CD5+ cases (80% v 11%; P < .01). Another myeloid marker, CD33, was expressed neither on CD5+ nor CD5- leukemic cells. CD22, a restricted B-cell marker, was expressed more frequently on CD5- leukemic cells than on CD5+ leukemic cells (80% v 33%; P < .05). Another restricted B-cell activation marker, CD23, was expressed at similar frequency in both the CD5+ and CD5- groups (67% v 60%). Although CD45RA was expressed on the majority of leukemic B cells, the CD45RA expression level was significantly higher among CD5- cases than CD5+ cases (P < .01). In the analysis of VH gene expressed in chronic B-cell leukemias by polymerase chain reaction amplification, CD5+ cases preferentially used VH4 family members (48%; 10 of 21). CD5- cases, on the other hand, mainly used VH3 family (80%; 4 of 5). Thus, from our present observation of an albeit limited patient population, we have found an association between VH gene family use and CD5 antigen expression in chronic B-cell leukemias. We have also shown the differential expression of myelomonocytic markers in the CD5+ and CD5- chronic B-cell leukemias. These result are in agreement with previous suggestions that CD5 positivity is the hallmark for distinct clinical entity commonly referred to in the literature as chronic lymphocytic leukemia.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
83
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2602-10
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7513207-Adult,
pubmed-meshheading:7513207-Aged,
pubmed-meshheading:7513207-Aged, 80 and over,
pubmed-meshheading:7513207-Antigens, CD,
pubmed-meshheading:7513207-Antigens, CD45,
pubmed-meshheading:7513207-Antigens, CD5,
pubmed-meshheading:7513207-Base Sequence,
pubmed-meshheading:7513207-Female,
pubmed-meshheading:7513207-Granulocytes,
pubmed-meshheading:7513207-Humans,
pubmed-meshheading:7513207-Immunoglobulin Heavy Chains,
pubmed-meshheading:7513207-Immunophenotyping,
pubmed-meshheading:7513207-Japan,
pubmed-meshheading:7513207-Leukemia, Lymphocytic, Chronic, B-Cell,
pubmed-meshheading:7513207-Male,
pubmed-meshheading:7513207-Middle Aged,
pubmed-meshheading:7513207-Molecular Sequence Data,
pubmed-meshheading:7513207-Monocytes,
pubmed-meshheading:7513207-Polymerase Chain Reaction
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Surface phenotype and Ig heavy-chain gene usage in chronic B-cell leukemias: expression of myelomonocytic surface markers in CD5- chronic B-cell leukemia.
|
| pubmed:affiliation |
First Department of Internal Medicine, Kyushu University, Fukuoka, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|