Linked Life Data

7512888

Source:http://linkedlifedata.com/resource/pubmed/id/7512888

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1994-5-23
pubmed:abstractText
The association of low doses of interleukin-2 (IL-2; 5 IU/ml) and interferon beta (IFN beta; 10 IU/ml) induced an additive or synergic stimulatory effect on natural killer (NK) activity (32%) in peripheral blood samples from hairy-cell leukemia patients, both those with active disease and those in remission. The synergic NK stimulatory effect was more commonly found in samples from patients with active disease, while the additive effect was more frequent in the patients in remission. The IL-2/IFN beta combination provoked a nonadditive nonsynergic NK-stimulatory effect in a further 19.8% samples. The targets of the IL-2/IFN beta combination were typical NK cells, as shown by the fact that there was increased cytotoxicity (synergic, additive or nonadditive nonsynergic) against the K562, but not the Daudi cell line in peripheral blood mononuclear cell samples treated with the combination of the two cytokines. When CD16+/CD56+ or CD57+/CD16+/CD56+ cells were removed, the NK-stimulatory effect was lost. The fact that the NK-cell-enhancing activity of the IL-2/IFN beta combination was reduced when Percoll fractions 2 and 3 were used, but still persisted in 66% of tests, may have been due to cytotoxicity being higher in the untreated fractions 2 and 3 than in the untreated unfractionated samples. One of the factors responsible for the NK-stimulatory effect appears to be the capacity of the IL-2/IFN beta combination to trigger an increase in IFN gamma synthesis. If similar experiments give like results in samples from patients suffering from other B-cell lymphoproliferative, or HIV-associated disorders, all of which are characterized by a deficiency in NK activity, it should be possible to use low-dose IL-2/IFN beta to treat these disorders and, perhaps, residual neoplastic disease without exposing the patient to undue toxicity. Further, by testing other combinations one should be able to identify the lowest IL-2 and IFN beta doses that would effectively boost the additive or synergic effect in a greater number of cases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0340-7004
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
323-31
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:7512888-Antigens, CD, pubmed-meshheading:7512888-Antigens, CD56, pubmed-meshheading:7512888-Antigens, CD57, pubmed-meshheading:7512888-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:7512888-Cytotoxicity, Immunologic, pubmed-meshheading:7512888-Drug Synergism, pubmed-meshheading:7512888-Drug Therapy, Combination, pubmed-meshheading:7512888-Humans, pubmed-meshheading:7512888-Immunophenotyping, pubmed-meshheading:7512888-Interferon-alpha, pubmed-meshheading:7512888-Interferon-beta, pubmed-meshheading:7512888-Interleukin-2, pubmed-meshheading:7512888-Killer Cells, Natural, pubmed-meshheading:7512888-Leukemia, Hairy Cell, pubmed-meshheading:7512888-Receptors, IgG, pubmed-meshheading:7512888-Recombinant Proteins, pubmed-meshheading:7512888-Remission Induction, pubmed-meshheading:7512888-Tumor Cells, Cultured
pubmed:year
1994
pubmed:articleTitle
Natural-killer-stimulatory effect of combined low-dose interleukin-2 and interferon beta in hairy-cell leukemia patients.
pubmed:affiliation
Istituto Medicina Interna e Scienze Oncologiche, Policlinico Monteluce, Perugia, Italy.
pubmed:publicationType
Journal Article