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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1994-5-16
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pubmed:abstractText |
CD28 is a costimulatory receptor that can provide the second signal necessary for T-cell activation and function in response to stimulation through the T-cell antigen receptor/CD3 complex. We found that a distinct array of proteins was phosphorylated on tyrosine following stimulation with anti-CD28 monoclonal antibody, as detected by immune-complex kinase assays. Anti-CD28 stimulation of in vitro kinase activity was detergent-dependent, occurring in immune complexes prepared with Brij 96 but not Nonidet P-40. Pretreatment of cells with low concentrations of phorbol ester increased the activation-independent phosphorylation of proteins in CD28 immune complexes. Reimmunoprecipitation studies indicated that the cytoplasmic protein-tyrosine kinases Lck and Fyn were associated with CD28. CD28 itself was phosphorylated both in vitro and in vivo in an activation-dependent manner, as detected by nonreducing/reducing SDS/PAGE analyses. The activation-stimulated phosphorylation of CD28 may play a key role in signaling through this receptor.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-1318900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-1335362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-1372649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-1846320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-1848158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-2155264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-2156582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-3041347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-3159820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-3487028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-3920341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-6235110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-6547160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-667938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-7694153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-7694363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-8386518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7512728-8476569
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigen-Antibody Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Detergents,
http://linkedlifedata.com/resource/pubmed/chemical/FYN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Specific Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fyn,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
91
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pubmed:geneSymbol |
fyn,
lyn
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3260-4
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7512728-Antigen-Antibody Complex,
pubmed-meshheading:7512728-Antigens, CD28,
pubmed-meshheading:7512728-Detergents,
pubmed-meshheading:7512728-Humans,
pubmed-meshheading:7512728-Lymphocyte Activation,
pubmed-meshheading:7512728-Lymphocyte Specific Protein Tyrosine Kinase p56(lck),
pubmed-meshheading:7512728-Macromolecular Substances,
pubmed-meshheading:7512728-Phosphoproteins,
pubmed-meshheading:7512728-Phosphotyrosine,
pubmed-meshheading:7512728-Protein Binding,
pubmed-meshheading:7512728-Protein-Tyrosine Kinases,
pubmed-meshheading:7512728-Proto-Oncogene Proteins,
pubmed-meshheading:7512728-Proto-Oncogene Proteins c-fyn,
pubmed-meshheading:7512728-T-Lymphocytes,
pubmed-meshheading:7512728-Tetradecanoylphorbol Acetate,
pubmed-meshheading:7512728-Tyrosine
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pubmed:year |
1994
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pubmed:articleTitle |
Activation-dependent phosphorylation of the T-lymphocyte surface receptor CD28 and associated proteins.
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pubmed:affiliation |
Division of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA 02115.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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