Linked Life Data

7512367

Source:http://linkedlifedata.com/resource/pubmed/id/7512367

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1994-5-13
pubmed:abstractText
Leukemic cells from two patients with Philadelphia-negative chronic myeloid leukemia (CML) were investigated: 1) Cytogenetics showed a normal 46,XY karyotype in both cases, 2) molecular studies revealed rearrangement of the M-BCR region and formation of BCR-ABL fusion mRNA with b2a2 (patient 1) or b3a2 (patient 2) configuration, and 3) fluorescence in situ hybridization (FISH) demonstrated relocation of the 5' BCR sequences from one chromosome 22 to one chromosome 9. The ABL probe hybridized to both chromosomes 9 at band q34, while two other probes which map centromeric and telomeric of BCR on 22q11 hybridized solely with chromosome 22. For the first time, a BCR-ABL rearrangement is shown to take place on 9q34 instead of in the usual location on 22q11. A rearrangement in the latter site is found in all Ph-positive CML and in almost all investigated CML with variant Ph or Ph-negative, BCR-positive cases. The few aberrant chromosomal localizations of BCR-ABL recombinant genes found previously were apparently the result of complex and successive changes. Furthermore in patient 2, both chromosomes 9 showed positive FISH signals with both ABL and BCR probes. Restriction fragment length polymorphism (RFLP) analysis indicated that mitotic recombination had occurred on the long arm of chromosome 9 and that the rearranged chromosome 9 was of paternal origin. The leukemic cells of this patient showed a duplication of the BCR-ABL gene, analogous to duplication of the Ph chromosome in classic CML. In addition they had lost the maternal alleles of the 9q34 chromosomal region.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1045-2257
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:geneSymbol
ABL, CR
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-45
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:7512367-Adult, pubmed-meshheading:7512367-Blotting, Southern, pubmed-meshheading:7512367-Chromosomes, Human, Pair 22, pubmed-meshheading:7512367-Chromosomes, Human, Pair 9, pubmed-meshheading:7512367-Fatal Outcome, pubmed-meshheading:7512367-Fusion Proteins, bcr-abl, pubmed-meshheading:7512367-Gene Rearrangement, pubmed-meshheading:7512367-Humans, pubmed-meshheading:7512367-In Situ Hybridization, Fluorescence, pubmed-meshheading:7512367-Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative, pubmed-meshheading:7512367-Male, pubmed-meshheading:7512367-Middle Aged, pubmed-meshheading:7512367-Mitosis, pubmed-meshheading:7512367-Oncogenes, pubmed-meshheading:7512367-Polymorphism, Restriction Fragment Length, pubmed-meshheading:7512367-Recombination, Genetic, pubmed-meshheading:7512367-Translocation, Genetic
pubmed:year
1993
pubmed:articleTitle
Translocation of BCR to chromosome 9: a new cytogenetic variant detected by FISH in two Ph-negative, BCR-positive patients with chronic myeloid leukemia.
pubmed:affiliation
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
pubmed:publicationType
Journal Article, Case Reports, Research Support, Non-U.S. Gov't