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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
14
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pubmed:dateCreated |
1994-5-5
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L27556,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L27557,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L27558,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L27559,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L27560
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pubmed:abstractText |
To better understand the regulation of insulin-like growth factor binding protein-5 (IGFBP-5) expression, we cloned the IGFBP-5 gene from human genomic libraries and identified a region in the 5' flanking sequence which functions as a promoter. The human IGFBP-5 gene is divided into four exons which, primarily due to a first intron of approximately 25 kilobases, span approximately 33 kilobases of DNA. Southern analysis identified a single copy of the IGFBP-5 gene in the haploid human genome, and several independent mapping strategies found this gene tightly linked with, and in opposite transcriptional orientation to, the IGFBP-2 gene at chromosomal region 2q33-34. Primer extension studies identified the IGFBP-5 mRNA cap site 772 base pairs (bp) 5' to the first nucleotide of the translation start codon. Analysis of the 5'-flanking sequence identified a potential TATA element beginning 33 bp 5' to the mRNA cap site. When a DNA fragment containing this cap site and 461 bp of upstream sequence was placed 5' to the chloramphenicol acetyltransferase reporter gene and transfected into MDA-MB-468 human breast cancer cells, it directed chloramphenicol acetyltransferase expression in an orientation-specific manner, suggesting that this region contains elements essential for IGFBP-5 promoter activity.
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pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Somatomedins
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
|
pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
8
|
pubmed:volume |
269
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pubmed:geneSymbol |
IGFBP-5
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
10891-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7511611-Amino Acid Sequence,
pubmed-meshheading:7511611-Base Sequence,
pubmed-meshheading:7511611-Carrier Proteins,
pubmed-meshheading:7511611-Cells, Cultured,
pubmed-meshheading:7511611-Chromosome Mapping,
pubmed-meshheading:7511611-Chromosomes, Human,
pubmed-meshheading:7511611-DNA, Complementary,
pubmed-meshheading:7511611-Humans,
pubmed-meshheading:7511611-Insulin-Like Growth Factor Binding Protein 5,
pubmed-meshheading:7511611-Molecular Sequence Data,
pubmed-meshheading:7511611-Promoter Regions, Genetic,
pubmed-meshheading:7511611-RNA, Messenger,
pubmed-meshheading:7511611-Somatomedins
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pubmed:year |
1994
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pubmed:articleTitle |
Characterization of the chromosomal gene and promoter for human insulin-like growth factor binding protein-5.
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pubmed:affiliation |
Department of Clinical Genetics, Karolinska Institute, Stockholm, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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