Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1994-4-28
pubmed:abstractText
Src homology 2 (SH2) domains provide specificity to intracellular signaling by binding to specific phosphotyrosine (phospho-Tyr)-containing sequences. We recently developed a technique using a degenerate phosphopeptide library to predict the specificity of individual SH2 domains (src family members, Abl, Nck, Sem5, phospholipase C-gamma, p85 subunit of phosphatidylinositol-3-kinase, and SHPTP2 (Z. Songyang, S. E. Shoelson, M. Chaudhuri, G. Gish, T. Pawson, W. G. Haser, F. King, T. Roberts, S. Ratnofsky, R. J. Lechleider, B. G. Neel, R. B. Birge, J. E. Fajardo, M. M. Chou, H. Hanafusa, B. Schaffhausen, and L. C. Cantley, Cell 72:767-778, 1993). We report here the optimal recognition motifs for SH2 domains from GRB-2, Drk, Csk, Vav, fps/fes, SHC, Syk (carboxy-terminal SH2), 3BP2, and HCP (amino-terminal SH2 domain, also called PTP1C and SHPTP1). As predicted, SH2 domains from proteins that fall into group I on the basis of a Phe or Tyr at the beta D5 position (GRB-2, 3BP2, Csk, fps/fes, Syk C-terminal SH2) select phosphopeptides with the general motif phospho-Tyr-hydrophilic (residue)-hydrophilic (residue)-hydrophobic (residue). The SH2 domains of SHC and HCP (group III proteins with Ile, Leu, of Cys at the beta D5 position) selected the general motif phospho-Tyr-hydrophobic-Xxx-hydrophobic, also as predicted. Vav, which has a Thr at the beta D5 position, selected phospho-Tyr-Met-Glu-Pro as the optimal motif. Each SH2 domain selected a unique optimal motif distinct from motifs previously determined for other SH2 domains. These motifs are used to predict potential sites in signaling proteins for interaction with specific SH2 domain-containing proteins. The Syk SH2 domain is predicted to bind to Tyr-hydrophilic-hydrophilic-Leu/Ile motifs like those repeated at 10-residue intervals in T- and B-cell receptor-associated proteins. SHC is predicted to bind to a subgroup og these same motifs. A structural basis for the association of Csk with Src family members is also suggested from these studies.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1311423, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1314163, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1370584, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1371171, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1372092, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1372437, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1374684, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1396585, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-15335710, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1537335, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1623525, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1696179, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1703628, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1717999, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1760844, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1846320, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1874735, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-1968060, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-2156626, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-2166824, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-2173144, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-2441878, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-2472219, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-2477241, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-2480346, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-2550144, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-2833705, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-2927501, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-2987699, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-3056717, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-3329718, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-7678249, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-7680435, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-7680959, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-7680960, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-7682895, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-7683653, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-7684160, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-7684496, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-7688466, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-7691811, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-7692233, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-8226808, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-8235613, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-8438166, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-8462098, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-8484124, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-8491186, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511210-8500161
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
2777-85
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
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