Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1994-4-25
pubmed:abstractText
Retroviruses, as a result of the presence of two identical genomic RNA molecules in their virions, recombine at a high rate. When nonhomologous RNA is present in the dimer RNA molecules, nonhomologous recombination can occur, although the rate is very low, only 0.1% of the rate of essentially homologous recombination (J. Zhang and H. M. Temin, Science 259:234-238, 1993). We found, as is found in naturally occurring highly oncogenic retroviruses (J. Zhang and H. M. Temin, J. Virol. 67:1747-1751, 1993), that the crossovers usually occur at a short region of sequence identity. We modified the previously studied vectors to study the effect of different lengths of short regions of sequence identity in the midst of otherwise nonidentical sequences. We found that the efficiency of recombination depends on the length of this sequence identity. However, the highest rate in such molecules remained lower than for recombination between essentially homologous molecules, even when there was extensive sequence identity. Junction sequences of the recombinants indicated that retrovirus recombination is not an error-prone process as was reported for human immunodeficiency virus reverse transcriptase by using a cell-free system (J. A. Peliska and S. J. Benkovic, Science 258:1112-1118, 1992).
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7511170-1279806, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511170-1371365, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511170-1850008, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511170-2031285, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511170-215703, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511170-2292186, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511170-2304918, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511170-2631796, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511170-3796606, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511170-7688465, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511170-8421784, http://linkedlifedata.com/resource/pubmed/commentcorrection/7511170-8445707
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2409-14
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Retrovirus recombination depends on the length of sequence identity and is not error prone.
pubmed:affiliation
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison 53706.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.