Linked Life Data

7510582

Source:http://linkedlifedata.com/resource/pubmed/id/7510582

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-4-19
pubmed:abstractText
We have examined whether aging is accompanied by changes in the capacity of CD4+ cells to produce IL-10, a potent immunoregulatory cytokine. Splenic CD4+ cells from young-adult and old C57BL/6NNia mice were stimulated in vitro with immobilized anti-CD3 epsilon mAb and were monitored for the release of IL-10 in short-term (3-day) cultures. In both age groups, detectable IL-10 accumulation in culture supernatants was stimulation dependent and reached a maximum level on Day 3. However, the peak IL-10 level in the old group was approximately 10-fold higher than that in the young-adult group. This age-associated difference in IL-10 production was also evident in the analysis of IL-10 mRNA levels in stimulated CD4+ cells. In contrast to these findings, the analysis of S-phase activity in the stimulated cell cultures revealed an age-related decline in this aspect of the cellular response. In studies on CD4+CD44lo and CD4+CD44hi subsets isolated from mice of various ages, we found that measurable IL-10 production segregated entirely with the CD44hi population, regardless of donor age. Taken together, our data suggest that the capacity for IL-10 synthesis by the splenic CD4+ cell pool is increased with age, and that the age-related shift toward a predominance of CD4+CD44hi cells in the peripheral tissues accounts for this quantitative change in IL-10 gene expression.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0008-8749
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
154
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
264-72
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Interleukin-10 production by splenic CD4+ cells and cell subsets from young and old mice.
pubmed:affiliation
Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't