Linked Life Data

7510251

Source:http://linkedlifedata.com/resource/pubmed/id/7510251

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-4-12
pubmed:abstractText
SV40 T antigen can induce senescent human diploid fibroblasts to synthesize DNA; however, the cells fail to go through mitosis. In this study, we examined the expression of the cdc2 and cyclin B genes, which are required for completion of mitosis, to determine whether defects in their expression occurred when SV40-transformed human cells entered the phase of crisis. If defects were observed it would indicate that immortalization by the virus involved reexpression of these genes. We found that the expression of cdc2 was unimpaired at both the RNA and protein levels, but that cyclin B expression was decreased in cells in crisis when compared with precrisis (mortal) and postcrisis (immortal) cells. Tritiated thymidine uptake demonstrated that the majority of cells in crisis were not actively cycling. Consistent with the latter observation we found that cyclin A, which is required for cells to traverse through S to G2, was downregulated in these cells. Since many of the results obtained with cells in crisis were similar to what is observed in normal human cells when they become senescent, we analyzed the expression of the genes fibronectin and sdi1 (a gene recently cloned from senescent cells that codes for an inhibitor of DNA synthesis). Both genes were overexpressed in cells during crisis, as is the case with senescent cells. The results are discussed in terms of the two-stage model previously proposed to explain the process of immortalization of human diploid fibroblasts by SV40.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0014-4827
pubmed:author
pubmed:issnType
Print
pubmed:volume
211
pubmed:geneSymbol
cdc2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
82-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
SV40-transformed human cells in crisis exhibit changes that occur in normal cellular senescence.
pubmed:affiliation
Roy M. and Phyllis Gough, Huffington Center on Aging, Baylor College of Medicine, Houston, Texas 77030.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.