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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1994-3-15
|
| pubmed:abstractText |
The antibody recognition of linear regions within the amino acid (aa) sequence of hepatitis B (HB) core antigen (HBcAg), e antigen (HBeAg), and pre-C region was investigated in 46 patients infected with hepatitis B virus (HBV), and one immunized rabbit. Peptide analogues were synthesized to cover the complete product of the C-gene, including the pre-C region using various synthetic methods. Two carriers of hepatitis B surface antigen (HBsAg) with anti-HBe, recognized pin-bound decapeptides covering amino acid (aa) 76-83 of HBc/eAg, and the most essential residues were found to be Asp78, Pro79, Arg82, and Asp83. Pre-C peptides were recognized by IgG1 or IgG3 in sera from two out of ten cases with acute HB, in four out of twelve sera from HBeAg-positive carriers of HBsAg, and in two out of twelve sera from anti-HBe-positive carriers of HBsAg. Two sera from the cases of acute HB showed strong reactivity of the IgG3 isotype with HBc/eAg peptides 61-85. Five of the sera from HBeAg-positive carriers of HBsAg were weakly reactive with peptides 41-60, 61-85, 121-140, and/or 141-160. Eight of the sera from anti-HBe-positive carriers of HBsAg recognized aa 121-140 of HBc/e with IgG1, IgG3, and/or IgG4 isotypes. IgG from one immunized rabbit recognized peptides 1-20, 61-85, and 71-90, and the T-cells recognized peptides 1-20 and 71-90. Thus, human and rabbit antibodies recognize linear antigenic regions within the pre-C, and within regions 1-20, 41-60, 61-85, 121-140, and 141-160 of HBcAg.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B Core Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B Surface Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B e Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0146-6615
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
42
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
7-15
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7508491-Amino Acid Sequence,
pubmed-meshheading:7508491-Animals,
pubmed-meshheading:7508491-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:7508491-Epitopes,
pubmed-meshheading:7508491-Genes, Viral,
pubmed-meshheading:7508491-Hepatitis B,
pubmed-meshheading:7508491-Hepatitis B Antibodies,
pubmed-meshheading:7508491-Hepatitis B Core Antigens,
pubmed-meshheading:7508491-Hepatitis B Surface Antigens,
pubmed-meshheading:7508491-Hepatitis B e Antigens,
pubmed-meshheading:7508491-Hepatitis B virus,
pubmed-meshheading:7508491-Humans,
pubmed-meshheading:7508491-Immunoglobulin G,
pubmed-meshheading:7508491-Lymphocyte Activation,
pubmed-meshheading:7508491-Male,
pubmed-meshheading:7508491-Molecular Sequence Data,
pubmed-meshheading:7508491-Oligopeptides,
pubmed-meshheading:7508491-Peptide Chain Initiation, Translational,
pubmed-meshheading:7508491-Protein Biosynthesis,
pubmed-meshheading:7508491-Rabbits,
pubmed-meshheading:7508491-Radioimmunoassay,
pubmed-meshheading:7508491-Sequence Homology, Amino Acid
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Immune recognition of linear antigenic regions within the hepatitis B pre-C and C-gene translation products using synthetic peptides.
|
| pubmed:affiliation |
Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
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| pubmed:publicationType |
Journal Article
|