Linked Life Data

7508473

Source:http://linkedlifedata.com/resource/pubmed/id/7508473

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-3-17
pubmed:abstractText
We propose a general solution to the problem of using antibodies originating in the same species for double immunohistochemical labeling. It relies on the use of a two-step protocol in which a secondary polyclonal monovalent F(ab) antibody present in the first step blocks access in the second of the secondary antibody to the primary antibody, which is continuously present from the first step. The monovalence of the F(ab) fragment eliminates the possibility of its linking the primary antibody from the second step. We designed two efficiency tests to explore the limits of the method by the very sensitive chemiluminescent system applied to sections of human pituitary tissue. They confirmed both the validity of the method and the necessity of adapting working conditions to obtain a complete lack of interference.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1554
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
433-7
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
F(ab) secondary antibodies: a general method for double immunolabeling with primary antisera from the same species. Efficiency control by chemiluminescence.
pubmed:affiliation
INSERM U244, DBMS/BRCE, CHRU Grenoble, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't