Linked Life Data

7507108

Source:http://linkedlifedata.com/resource/pubmed/id/7507108

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-2-22
pubmed:abstractText
Previously we have shown that high metastatic colonic carcinoma cells express relatively more lamp molecules and sialyl Le(x) structures on the cell surface than their corresponding low metastatic counterparts (Saitoh, O., Wang, W.-L., Lotan, R., and Fukuda, M. (1992) J. Biol. Chem. 267, 5700-5711). In the present study, we extended these findings by testing whether these high and low metastatic colonic carcinoma cells differ in their adhesion efficiency to E-selectin-expressing cells. First, it was found that the high metastatic cells, as compared to their low metastatic counterparts, bind more efficiently to activated human endothelial cells that express E-selectin. This was also true when the adhesion was tested for Chinese hamster ovary cells stably expressing E-selectin. In addition, it was found that the high metastatic cells also adhere more efficiently to mouse endothelioma cells after activation with interleukin-1 beta. It was also shown that the adhesion can be inhibited by soluble lamp-1 or soluble leukosialin that contain sialy Le(x) termini. The inhibition was not, however, observed when these soluble glycoproteins lack sialyl Le(x) structures. The results indicate that the efficiency of the E-selectin-mediated binding of colonic carcinoma cells to human and mouse endothelial cells correlates with the metastatic potential of the cells and suggest that this adhesive event may be one of the critical factors for the metastatic spread of tumor cells. Soluble forms of leukosialin or lamp-1 may be useful as therapeutic agents for the inhibition of E-selectin-mediated binding to tumor cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
269
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1425-31
pubmed:dateRevised
2011-9-7
pubmed:meshHeading
pubmed-meshheading:7507108-Animals, pubmed-meshheading:7507108-Antigens, CD, pubmed-meshheading:7507108-Antigens, CD15, pubmed-meshheading:7507108-Antigens, CD43, pubmed-meshheading:7507108-Base Sequence, pubmed-meshheading:7507108-Carcinoma, pubmed-meshheading:7507108-Cell Adhesion, pubmed-meshheading:7507108-Cell Adhesion Molecules, pubmed-meshheading:7507108-Colonic Neoplasms, pubmed-meshheading:7507108-DNA Primers, pubmed-meshheading:7507108-E-Selectin, pubmed-meshheading:7507108-Endothelium, Vascular, pubmed-meshheading:7507108-Humans, pubmed-meshheading:7507108-Lysosome-Associated Membrane Glycoproteins, pubmed-meshheading:7507108-Membrane Glycoproteins, pubmed-meshheading:7507108-Mice, pubmed-meshheading:7507108-Molecular Sequence Data, pubmed-meshheading:7507108-Neoplasm Metastasis, pubmed-meshheading:7507108-Sialoglycoproteins
pubmed:year
1994
pubmed:articleTitle
Differential E-selectin-dependent adhesion efficiency in sublines of a human colon cancer exhibiting distinct metastatic potentials.
pubmed:affiliation
Glycobiology Program, La Jolla Cancer Research Foundation, California 92037.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't