Linked Life Data

7506598

Source:http://linkedlifedata.com/resource/pubmed/id/7506598

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-2-16
pubmed:abstractText
In order to study extrathymic differentiation in vitro, CD7+CD3- lymphocytes were sorted from normal human bone marrow and cultured under conditions of limiting dilution together with irradiated pooled allogeneic peripheral blood mononuclear cells (PBMC) and phytohemagglutinin (PHA) in the presence of 1000 U/ml of interleukin-2 (IL-2). One clone was obtained that failed to react with monoclonal antibody (mAb) TCR delta 1 (TCR1, gamma/delta-specific) or WT31 (TCR2, alpha/beta-specific). From day 35 through day 74 in culture, the surface phenotype of this clone evolved into CD3+, CD4+, CD8-, TCR2+, TCR1-, and was further characterized as CD2+, CD45RO+, CD16-, and CD56-. The presence of mRNA for TCR alpha and beta but not gamma and delta chains was confirmed by Northern blotting. Accessory cell-dependent autocrine proliferative responses to PHA (most likely driven by IL-2) were initially absent, but became measurable at the same time as the TCR was acquired. However, in the absence of PHA, the clone failed to respond to a panel of homozygous B-cell lines representing the majority of MHC class II alleles. Autoreactivity was also not demonstrable. Cytotoxicity was limited to MHC unrestricted "natural killer (NK)-like" lysis of K562 target cells, with no autocytotoxicity detected. The NK-like lysis diminished over time in parallel with the acquisition of surface TCR. The cloned cells were not suppressive for mature lymphocyte proliferation. After stimulation, the cells secreted tumor necrosis factor alpha and granulocyte/macrophage colony-stimulating factor (GM-CSF) detected by immunoassays, and T-cell growth factors, most likely IL-2, as detected by bioassays. Polymerase chain-reaction methods demonstrated the presence of mRNA for IL-2, IL-3, IL-4, IL-9, interferon-gamma, and GM-CSF in these cells after stimulation with PHA and B-LCL. These results suggest that cells with the phenotype and some functional characteristics of mature T lymphocytes can evolve extrathymically in vitro from T-cell precursors sorted from normal human bone marrow.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1044-6672
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
197-210
pubmed:dateRevised
2008-11-20
pubmed:meshHeading
pubmed-meshheading:7506598-Antigens, CD, pubmed-meshheading:7506598-Antigens, CD3, pubmed-meshheading:7506598-Antigens, CD4, pubmed-meshheading:7506598-Antigens, CD7, pubmed-meshheading:7506598-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:7506598-Base Sequence, pubmed-meshheading:7506598-Blotting, Northern, pubmed-meshheading:7506598-Blotting, Southern, pubmed-meshheading:7506598-Bone Marrow Cells, pubmed-meshheading:7506598-Cells, Cultured, pubmed-meshheading:7506598-Cytokines, pubmed-meshheading:7506598-Cytotoxicity Tests, Immunologic, pubmed-meshheading:7506598-Flow Cytometry, pubmed-meshheading:7506598-Humans, pubmed-meshheading:7506598-Lymphocyte Activation, pubmed-meshheading:7506598-Molecular Sequence Data, pubmed-meshheading:7506598-RNA, Messenger, pubmed-meshheading:7506598-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:7506598-T-Lymphocytes
pubmed:year
1993
pubmed:articleTitle
Evolution of a CD3+CD4+ alpha/beta T-cell receptor+ mature T-cell clone from CD3-CD7+ sorted human bone marrow cells.
pubmed:affiliation
Medical and Natural Sciences Research Center, University of Tübingen, Federal Republic of Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't