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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6 Pt 1
|
| pubmed:dateCreated |
1994-2-8
|
| pubmed:abstractText |
Guanosine 5'-cyclic monophosphate (cGMP) is an important modulator of fluid balance in many epithelia. We examined its metabolism in primary cultures of human airway epithelia. Sodium nitroprusside increased cGMP levels 30-fold, suggesting that the respiratory epithelium expresses a soluble guanylate cyclase; however, endogenous nitric oxide production was not detected. cGMP levels could also be increased by C-type natriuretic peptide (CNP), but not by atrial natriuretic peptide, brain natriuretic peptide, or Escherichia coli heat-stable enterotoxin, indicating expression of a CNP-specific membrane-bound guanylate cyclase. The one-half effective concentration for CNP was 40 nM and the maximal velocity was 56.7 pmol cGMP.mg protein-1.h-1. After CNP stimulation, approximately 60% of the total synthesized cGMP was preferentially exported from the polarized epithelial cells across the basolateral membrane by a probenecid-sensitive process. Isoproterenol-stimulated adenosine 3',5'-cyclic monophosphate (cAMP) export revealed a similar export pattern and probenecid sensitivity, although a lower efficiency of export (27% of total cAMP was exported). Consistent with previous reports, export of neither cyclic nucleotide was saturable at the concentrations tested. We conclude that the respiratory epithelium expresses a soluble guanylate cyclase, a CNP-specific receptor, and a novel vectorial cyclic nucleotide export mechanism.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-Methyl-3-isobutylxanthine,
http://linkedlifedata.com/resource/pubmed/chemical/Atrial Natriuretic Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic GMP,
http://linkedlifedata.com/resource/pubmed/chemical/Guanylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Natriuretic Peptide, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
265
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
L598-605
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7506495-1-Methyl-3-isobutylxanthine,
pubmed-meshheading:7506495-Adult,
pubmed-meshheading:7506495-Aged,
pubmed-meshheading:7506495-Animals,
pubmed-meshheading:7506495-Atrial Natriuretic Factor,
pubmed-meshheading:7506495-Cell Line,
pubmed-meshheading:7506495-Cell Membrane,
pubmed-meshheading:7506495-Cells, Cultured,
pubmed-meshheading:7506495-Cyclic AMP,
pubmed-meshheading:7506495-Cyclic GMP,
pubmed-meshheading:7506495-Dose-Response Relationship, Drug,
pubmed-meshheading:7506495-Epithelium,
pubmed-meshheading:7506495-Female,
pubmed-meshheading:7506495-Gene Expression,
pubmed-meshheading:7506495-Guanylate Cyclase,
pubmed-meshheading:7506495-Humans,
pubmed-meshheading:7506495-Isoenzymes,
pubmed-meshheading:7506495-Kinetics,
pubmed-meshheading:7506495-Lung,
pubmed-meshheading:7506495-Male,
pubmed-meshheading:7506495-Middle Aged,
pubmed-meshheading:7506495-Natriuretic Peptide, C-Type,
pubmed-meshheading:7506495-Nerve Tissue Proteins,
pubmed-meshheading:7506495-Rats,
pubmed-meshheading:7506495-Time Factors
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Synthesis and vectorial export of cGMP in airway epithelium: expression of soluble and CNP-specific guanylate cyclases.
|
| pubmed:affiliation |
Departments of Medicine and Physiology, University of North Carolina at Chapel Hill 27599.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|