Linked Life Data

7506491

Source:http://linkedlifedata.com/resource/pubmed/id/7506491

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6 Pt 1
pubmed:dateCreated
1994-2-8
pubmed:abstractText
Endurance exercise training elicits an increase in mitochondrial density as well as GLUT-4 glucose transporter protein content in skeletal muscle. Corresponding increases in mRNA for respiratory enzymes and GLUT-4 indicate that pretranslational control mechanisms are involved in this adaptive process. To directly test whether transcription of the GLUT-4 gene is activated in response to exercise training, nuclei were isolated from red hindlimb skeletal muscle of rats after 1 wk of exercise training (8% grade, 32 m/min, 40 min, twice/day). Rats were killed either 30 min, 3 h, or 24 h after the last training session. GLUT-4 transcription, determined by nuclear run-on analysis, was unaltered after 30 min, increased by 1.8-fold after 3 h, but was no longer different from controls 24 h after exercise. A similar transient increase in GLUT-4 transcription was evident, but less pronounced (1.4-fold), in untrained rats after a single bout of exercise, suggesting that the postexercise induction in GLUT-4 gene transcription is enhanced by exercise training. GLUT-4 protein content was increased 1.7-fold after 1 wk of training in the absence of any corresponding change in GLUT-4 mRNA, providing evidence that the initial increase in GLUT-4 expression involves translational and/or posttranslational control mechanisms. These findings demonstrate that muscle GLUT-4 expression in response to exercise training is subject to both transcriptional and posttranscriptional regulation. We propose that the increase in GLUT-4 mRNA evident with extended periods of training may result from a shift to pretranslational control and is the cumulative effect of repeated postexercise transient increases in GLUT-4 gene transcription.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
265
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C1597-603
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7506491-Analysis of Variance, pubmed-meshheading:7506491-Animals, pubmed-meshheading:7506491-Blotting, Northern, pubmed-meshheading:7506491-Blotting, Western, pubmed-meshheading:7506491-Cell Nucleus, pubmed-meshheading:7506491-Citrate (si)-Synthase, pubmed-meshheading:7506491-Gene Expression Regulation, pubmed-meshheading:7506491-Glucose Transporter Type 1, pubmed-meshheading:7506491-Glucose Transporter Type 4, pubmed-meshheading:7506491-Male, pubmed-meshheading:7506491-Molecular Weight, pubmed-meshheading:7506491-Monosaccharide Transport Proteins, pubmed-meshheading:7506491-Muscle Proteins, pubmed-meshheading:7506491-Muscles, pubmed-meshheading:7506491-Physical Conditioning, Animal, pubmed-meshheading:7506491-RNA, pubmed-meshheading:7506491-Rats, pubmed-meshheading:7506491-Rats, Sprague-Dawley, pubmed-meshheading:7506491-Time Factors, pubmed-meshheading:7506491-Transcription, Genetic
pubmed:year
1993
pubmed:articleTitle
Exercise induces a transient increase in transcription of the GLUT-4 gene in skeletal muscle.
pubmed:affiliation
Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.